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Literature summary for 3.2.1.18 extracted from

  • Hsiao, Y.S.; Parker, D.; Ratner, A.J.; Prince, A.; Tong, L.
    Crystal structures of respiratory pathogen neuraminidases (2009), Biochem. Biophys. Res. Commun., 380, 467-471.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa
expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
free enzyme and in complex with N-acetylneuraminic acid or 2,3-dehydro-2-deoxy-N-acetylneuraminic acid, sitting drop vapor diffusion method, using 100 mM HEPES (pH 7.0) and 30% Jeffamine ED-2001 (pH 7.0), at 21°C Streptococcus pneumoniae
free enzyme, sitting drop vapor diffusion method, using 100 mM HEPES (pH 7.0), 5% tacsimate, 7% (w/v) PEG 5000MME, at 21°C Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
D79A inactive Pseudomonas aeruginosa
R198K inactive Pseudomonas aeruginosa
R260A inactive Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-2,3-dehydro-N-acetyl-D-neuraminic acid
-
 Streptococcus pneumoniae
N-acetylneuraminic acid
-
 Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-agarose column chromatography and gel filtration Pseudomonas aeruginosa
nickel-agarose column chromatography and gel filtration Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
NanA
-
 Streptococcus pneumoniae
NanPs
-
 Pseudomonas aeruginosa
neuraminidase
-
 Pseudomonas aeruginosa
neuraminidase
-
 Streptococcus pneumoniae