Cloned (Comment) | Organism |
---|---|
gene neu-1 located in the major histocompatibility complex locus, expression of wild-type and mutant in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G413A | site-directed mutagenesis, activity and localization in the cell similar to the wild-type enzyme | Homo sapiens |
L415A | site-directed mutagenesis, 30-50% reduced activity compared to the wild-type enzyme, no internalization due to impaired endocytosis, location in the plasma membrane and the cytosol | Homo sapiens |
Y412A | site-directed mutagenesis, 55-70% reduced activity compared to the wild-type enzyme, no internalization due to impaired endocytosis, location in the plasma membrane and the cytosol | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endosome | - |
Homo sapiens | 5768 | - |
lysosome | major part | Homo sapiens | 5764 | - |
membrane | - |
Homo sapiens | 16020 | - |
additional information | intracellular distribution, enzyme contains a signal for internalization in form of a C-terminal tetrapeptide Y412GTL415, Y412 and L415 are essential for endocytosis of the enzyme, mechanism, targeting from lysosomal membrane to endosomes via clathrin-coated pits, enzyme pattern in AP3-deficient cells | Homo sapiens | - |
- |
plasma membrane | T-lymphocyte surface, minor part | Homo sapiens | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme is required for cellular signaling and for the production of cytokine interleukin-4 by activated T-lymphocytes, inherited enzyme defiency results in sialidosis or galactosialidosis, both severe metaboic disorders associated with lysosomal storage of oligosaccharides and glycopeptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene neu-1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
T-lymphocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is required for cellular signaling and for the production of cytokine interleukin-4 by activated T-lymphocytes, inherited enzyme defiency results in sialidosis or galactosialidosis, both severe metaboic disorders associated with lysosomal storage of oligosaccharides and glycopeptides | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000-48000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
neuraminidase | - |
Homo sapiens |
sialidase | - |
Homo sapiens |