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Literature summary for 3.2.1.18 extracted from
- Variation in the divalent cation requirements of influenza A virus N2 neuraminidases (2003), J. Biochem., 134, 345-352.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
influenza A virus |  |
| Zn2+ | slight inhibition | influenza A virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | influenza A virus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates about 2fold, increase in initial reaction velocity, required | influenza A virus | |
| Mg2+ | activates about 2fold, increase in initial reaction velocity, required | influenza A virus | |
| additional information | decrease in activity through removal of divalent cations results in decreased immunogenicity, antibody response decreases by about 68-76%, 80% of the original activity is restored by addition of Ca2+ and Mg2+ | influenza A virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| influenza A virus | - |
type N2, several strains | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from several strains | influenza A virus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity of different strains in presence or absence of divalent cations, overview | influenza A virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
influenza A virus | 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NA | - |
influenza A virus |
| neuraminidase | - |
influenza A virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | influenza A virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
assay at | influenza A virus |
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Release 2023.1 (January 2023)
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