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Literature summary for 3.2.1.18 extracted from

  • Burg, M.; Muthing, J.
    Characterization of cytosolic sialidase from Chinese hamster ovary cells: part I: cloning and expression of soluble sialidase in Escherichia coli (2001), Carbohydr. Res., 330, 335-346.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression as glutathione-S-transferase fusion protein in Escherichia coli Cricetulus griseus

Inhibitors

Inhibitors Comment Organism Structure
Neu5Ac i.e. alpha-D-N-acetylneuraminic acid, competitive, 17% inhibition at 2 mM, 20% inhibition at 5 mM with substrate 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Cricetulus griseus
Neu5Ac2en i.e. 2-deoxy-2,3-dehydro-alpha-D-N-acetylneuraminic acid, competitive, 49% inhibition at 0.1 mM, 75% inhibition at 0.5 mM with substrate 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid Cricetulus griseus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Cricetulus griseus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 69000, recombinant fusion enzyme, SDS-PAGE, x * 43000, untagged enzyme, SDS-PAGE Cricetulus griseus
69000
-
x * 69000, recombinant fusion enzyme, SDS-PAGE, x * 43000, untagged enzyme, SDS-PAGE Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant glutathione-S-transferase fusion protein from Escherichia coli, by a one-step glutathione-affinity chromatography, 40fold Cricetulus griseus

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell
-
Cricetulus griseus
-
ovary
-
Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.999
-
purified recombinant enzyme Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
Cricetulus griseus 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid
-
?

Subunits

Subunits Comment Organism
? x * 69000, recombinant fusion enzyme, SDS-PAGE, x * 43000, untagged enzyme, SDS-PAGE Cricetulus griseus

Synonyms

Synonyms Comment Organism
sialidase
-
Cricetulus griseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cricetulus griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Cricetulus griseus