Literature summary for 3.2.1.18 extracted from

Wang, P.; Zhang, J.; Bian, H.; Wu, P.; Kuvelkar, R.; Kung, T.T.; Crawley, Y.; Egan, R.W.; Billah, M.M.
Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor (2004), Biochem. J., 380, 425-433.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	isozymes Neu-1 and Neu-3 are induced in T-cells by CD3/CD28 co-stimulation, Neu-3 is the major inducible form in stimulated T-cells, specificity of induction, overview	Homo sapiens

Cloned (Comment)	Organism
isozymes Neu-1 and Neu-3, DNA sequence determination and analysis, expression of the enzymes in COS-7 cells, stably in JURKAT cells, and as His-tagged proteins in Spodoptera frugiperda Sf9 insect cells	Homo sapiens

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Mus musculus	5764	-
lysosome	-	Homo sapiens	5764	-
plasma membrane	-	Mus musculus	5886	-
plasma membrane	-	Homo sapiens	5886	-

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ganglioside GD1a + H2O	Mus musculus	i.e. NeuAcalpha(2-3)-Galbeta(1-3)-GalNAcbeta(1-4)-NeuAcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer, isozyme Neu-3, no activity with Neu-1	ganglioside GM1 + sialic acid	-	?
ganglioside GD1a + H2O	Homo sapiens	i.e. NeuAcalpha(2-3)-Galbeta(1-3)-GalNAcbeta(1-4)-NeuAcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer, isozyme Neu-3, no activity with Neu-1	ganglioside GM1 + sialic acid	-	?

Organism	UniProt	Comment	Textmining
Homo sapiens	-	3 isozymes Neu-1, Neu-2, and Neu-3	-
Mus musculus	-	3 isozymes Neu-1, Neu-2, and Neu-3	-

Source Tissue	Comment	Organism	Textmining
heart	-	Homo sapiens	-
heart	low expression of Neu-2	Mus musculus	-
kidney	-	Mus musculus	-
kidney	low expression of Neu-2	Homo sapiens	-
liver	-	Mus musculus	-
liver	-	Homo sapiens	-
lymph node	-	Homo sapiens	-
lymph node	low expression of Neu-2	Mus musculus	-
monocyte	-	Mus musculus	-
monocyte	low expression of Neu-2 and Neu-3	Homo sapiens	-
additional information	tissue distribution of the 3 isozymes, Neu-1 is ubiquitously expressed at higher level, except for neutrophils	Homo sapiens	-
additional information	tissue distribution of the 3 isozymes, Neu-1 is ubiquitously expressed at higher level, except for skeletal muscle	Mus musculus	-
neutrophil	-	Mus musculus	-
neutrophil	low amount of Neu-1	Homo sapiens	-
skeletal muscle	low amount of Neu-1	Mus musculus	-
skeletal muscle	low expression of Neu-2	Homo sapiens	-
spleen	-	Homo sapiens	-
spleen	low expression of Neu-2	Mus musculus	-
T-cell	CD4+ T-cells,low expression of Neu-2	Homo sapiens	-
T-cell	low expression of Neu-2	Mus musculus	-
thymus	-	Mus musculus	-
thymus	-	Homo sapiens	-

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	quantification by RT-PCR	Mus musculus
additional information	-	quantification by RT-PCR	Homo sapiens

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O	-	Homo sapiens	4-methylumbelliferol + alpha-D-N-acetylneuraminic acid	-	?
4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O	isozyme Neu-1 and Neu-3	Mus musculus	4-methylumbelliferol + alpha-D-N-acetylneuraminic acid	-	?
ganglioside GD1a + H2O	-	Homo sapiens	ganglioside GM1 + sialic acid	-	?
ganglioside GD1a + H2O	i.e. NeuAcalpha(2-3)-Galbeta(1-3)-GalNAcbeta(1-4)-NeuAcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer, isozyme Neu-3, no activity with Neu-1	Mus musculus	ganglioside GM1 + sialic acid	-	?
ganglioside GD1a + H2O	i.e. NeuAcalpha(2-3)-Galbeta(1-3)-GalNAcbeta(1-4)-NeuAcalpha(2-3)-Galbeta(1-4)-Glcbeta(1-1)-Cer, isozyme Neu-3, no activity with Neu-1	Homo sapiens	ganglioside GM1 + sialic acid	-	?
additional information	ganglioside substrate specificity of the isozymes, overview	Mus musculus	?	-	?
additional information	ganglioside substrate specificity of the isozymes, overview	Homo sapiens	?	-	?