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Literature summary for 3.2.1.18 extracted from

  • Miyagi, T.; Tsuiki, S.
    Rat liver lysosomal sialidase: solubilization, substrate specificity and comparison with the cytosolic sialidase (1984), Eur. J. Biochem., 141, 75-81.
    View publication on PubMed
Search for more literature for 3.2.1.18

General Stability

General Stability Organism
intralysosomal sialidase is stabilized by leupeptin, bovine serum albumin and divalent cations such as Mn2+, Ca2+, Mg2+ Rattus norvegicus
labile towards freezing and thrawing Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.92
-
 sialyllactose intralysosomal sialidase Rattus norvegicus
2.38
-
 sialyllactose cytosolic sialidase Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
lysosome
-
 Rattus norvegicus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.9
-
 cytosolic Rattus norvegicus
165
-
 intralysosomal Rattus norvegicus

Storage Stability

Storage Stability Organism
0°C, 8 h, totally inactivated Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sialyllactose + H2O
-
 Rattus norvegicus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 sialyllactose as substrate Rattus norvegicus