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Literature summary for 3.2.1.177 extracted from
- Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli (2004), Protein Expr. Purif., 37, 170-179.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
alpha-D-xylopyranosyl fluoride | pH 7.0, 37°C | Escherichia coli |  |
| 0.52 | - |
4-nitrophenyl-alpha-D-xyloside | pH 7.0, 37°C | Escherichia coli | |
| 0.55 | - |
Isoprimeverose | pH 7.0, 37°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
6 * 88000, SDS-PAGE | Escherichia coli |
| 88079 | - |
6 * 88079, calculated from sequence | Escherichia coli |
| 530000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P31434 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.503 | - |
pH 7.0, 37°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-D-xyloside + H2O | - |
Escherichia coli | 4-nitrophenol + alpha-D-xylose | - |
? | |
| alpha-D-xylopyranosyl fluoride + H2O | - |
Escherichia coli | alpha-D-xylose + fluoride | - |
? | |
| isoprimeverose + H2O | - |
Escherichia coli | alpha-D-xylose + beta-D-glucose | - |
? | |
| additional information | the enzyme alos shows transglycosylation activity | Escherichia coli | ? | - |
? | |
| xyloglucan oligosaccharide + H2O | - |
Escherichia coli | alpha-D-xylose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 88000, SDS-PAGE | Escherichia coli |
| hexamer | 6 * 88079, calculated from sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YicI | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
15 min, stable up to | Escherichia coli |
| 55 | - |
15 min, about 70% loss of activity | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.173 | - |
4-nitrophenyl-alpha-D-xyloside | pH 7.0, 37°C | Escherichia coli | |
| 51.3 | - |
Isoprimeverose | pH 7.0, 37°C | Escherichia coli | |
| 195 | - |
alpha-D-xylopyranosyl fluoride | pH 7.0, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | pH 5.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | 10.1 | 4°C, 24 h, stable | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.33 | - |
4-nitrophenyl-alpha-D-xyloside | pH 7.0, 37°C | Escherichia coli | |
| 93.3 | - |
Isoprimeverose | pH 7.0, 37°C | Escherichia coli | |
| 1220 | - |
alpha-D-xylopyranosyl fluoride | pH 7.0, 37°C | Escherichia coli | |
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