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Literature summary for 3.2.1.177 extracted from
- Cloning and expression pattern of a gene encoding an alpha-xylosidase active against xyloglucan oligosaccharides from Arabidopsis (2001), Plant Physiol., 126, 910-920.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the gene is introduced in a suitable expression vector and used to transform Saccharomyces cerevisiae | Arabidopsis thaliana |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 89000 | - |
x * 89000, SDS-PAGE | Brassica oleracea var. capitata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9S7Y7 | - |
- |
| Brassica oleracea var. capitata | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | contains eight possible glycosylation sites | Arabidopsis thaliana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Brassica oleracea var. capitata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Brassica oleracea var. capitata | - |
| leaf | alpha-xylosidase activity and AtXYL1 expression increase from older to younger leaves | Arabidopsis thaliana | - |
| seedling | apoplastic extraction | Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.7 | - |
pH 4.5, 40°C | Brassica oleracea var. capitata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | xyloglucan oligosaccharide from tamarind seed xyloglucan | Brassica oleracea var. capitata | xylose + ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 89000, SDS-PAGE | Brassica oleracea var. capitata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtXYL1 | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Brassica oleracea var. capitata |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Brassica oleracea var. capitata | isoelectric focusing | - |
8.3 |
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Release 2023.1 (January 2023)
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