Literature summary for 3.2.1.177 extracted from

Lovering, A.L.; Lee, S.S.; Kim, Y.W.; Withers, S.G.; Strynadka, N.C.
Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate (2005), J. Biol. Chem., 280, 2105-2115.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method, 2.2 A resolution structure	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
(5R)-5-fluoro-alpha-D-xylopyranosyl fluoride	mechanism-based inactivator	Escherichia coli
(5S)-5-fluoro-alpha-D-xylopyranosyl fluoride	mechanism-based inactivator	Escherichia coli
1-deoxynojirimycin	-	Escherichia coli
1-deoxyxylonojirimycin	-	Escherichia coli
xyloisofagomine	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.71	-	4-nitrophenyl-alpha-D-xyloside	pH 7.0, 37°C	Escherichia coli
0.97	-	alpha-D-xylopyranosyl fluoride	pH 7.0, 37°C	Escherichia coli
7.7	-	4-nitrophenyl-alpha-D-glucopyranoside	pH 7.0, 37°C	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no effect on hydrolysis of 4-nitrophenyl-alpha-D-xyloside: Ca2+, Mg2+, Mn2+, Zn2+, Ni2+, Cu2+, and Co2+	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P31434	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Storage Stability

Storage Stability	Organism
4°C, pH 7.0, retains full activity for several months	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O	low activity	Escherichia coli	4-nitrophenol + alpha-D-glucose	-	?
4-nitrophenyl-alpha-D-xyloside + H2O	-	Escherichia coli	4-nitrophenol + alpha-D-xylose	-	?
alpha-D-xylopyranosyl fluoride + H2O	-	Escherichia coli	alpha-D-xylose + fluoride	-	?

Subunits

Subunits	Comment	Organism
hexamer	crystallographic data	Escherichia coli

Synonyms

Synonyms	Comment	Organism
YicI	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Escherichia coli
50	-	hydrolysis of 4-nitrophenyl-alpha-D-xyloside increases up to 50°C, activity is rapidly lost at higher temperatures	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
7	-	37°C, retains full activity for 48 h	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.046	-	4-nitrophenyl-alpha-D-glucopyranoside	pH 7.0, 37°C	Escherichia coli
0.13	-	4-nitrophenyl-alpha-D-xyloside	pH 7.0, 37°C	Escherichia coli
75	-	alpha-D-xylopyranosyl fluoride	pH 7.0, 37°C	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.4	-	hydrolysis of 4-nitrophenyl-alpha-D-xyloside	Escherichia coli
7	-	assay at	Escherichia coli

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	pH 5.0: about 60% of maximal activity, pH 8.0: about 45% of maximal activity	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00045	-	(5R)-5-fluoro-alpha-D-xylopyranosyl fluoride	pH 7.0, 37°C, mechanism-based inactivator	Escherichia coli
0.0009	-	1-deoxyxylonojirimycin	pH 7.0, 37°C	Escherichia coli
0.0098	-	(5S)-5-fluoro-alpha-D-xylopyranosyl fluoride	pH 7.0, 37°C, mechanism-based inactivator	Escherichia coli
0.13	-	1-deoxynojirimycin	pH 7.0, 37°C	Escherichia coli
0.17	-	xyloisofagomine	pH 7.0, 37°C	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0064	-	4-nitrophenyl-alpha-D-glucopyranoside	pH 7.0, 37°C	Escherichia coli
0.18	-	4-nitrophenyl-alpha-D-xyloside	pH 7.0, 37°C	Escherichia coli
77.3	-	alpha-D-xylopyranosyl fluoride	pH 7.0, 37°C	Escherichia coli

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Release 2023.1 (January 2023)