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Literature summary for 3.2.1.177 extracted from
- Identification and molecular characterization of the first alpha-xylosidase from an archaeon (2000), J. Biol. Chem., 275, 22082-22089.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Saccharolobus solfataricus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
1 * 72000, SDS-PAGE | Saccharolobus solfataricus |
| 84432 | - |
1 * 84432, calculated from sequence | Saccharolobus solfataricus |
| 84500 | - |
mass spectrometry | Saccharolobus solfataricus |
| 92000 | - |
gel filtration | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q9P999 | - |
- |
| Saccharolobus solfataricus MT4 | Q9P999 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.43 | - |
pH 5.5, 75°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-xyloside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + alpha-D-xylose | - |
? |  |
| 4-nitrophenyl alpha-maltoside + H2O | low activity | Saccharolobus solfataricus | 4-nitrophenyl alpha-D-glucoside + D-glucose | - |
? | |
| 4-nitrophenyl alpha-maltoside + H2O | low activity | Saccharolobus solfataricus MT4 | 4-nitrophenyl alpha-D-glucoside + D-glucose | - |
? | |
| 4-nitrophenyl beta-isoprimeveroside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenyl beta-D-glucoside + D-xylose | - |
? | |
| 4-nitrophenyl-alpha-D-glucoside + H2O | kcat/KM is 0.2% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Saccharolobus solfataricus | 4-nitrophenol + alpha-D-glucose | - |
? | |
| 4-nitrophenyl-alpha-D-glucoside + H2O | kcat/KM is 0.2% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Saccharolobus solfataricus MT4 | 4-nitrophenol + alpha-D-glucose | - |
? | |
| 4-nitrophenyl-alpha-D-xyloside + H2O | kcat/KM is 3% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Saccharolobus solfataricus | 4-nitrophenol + alpha-D-xylose | - |
? | |
| 4-nitrophenyl-alpha-D-xyloside + H2O | kcat/KM is 3% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Saccharolobus solfataricus MT4 | 4-nitrophenol + alpha-D-xylose | - |
? | |
| isoprimeverose + H2O | kcat/KM is 11.5% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Saccharolobus solfataricus | alpha-D-xylose + beta-D-glucose | - |
? | |
| maltose + H2O | kcat/KM is 1% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Saccharolobus solfataricus | 2 D-glucose | - |
? | |
| maltose + H2O | kcat/KM is 1% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Saccharolobus solfataricus MT4 | 2 D-glucose | - |
? | |
| maltotriose + H2O | kcat/KM is 3% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Saccharolobus solfataricus | D-maltose + D-glucose | - |
? | |
| additional information | exo-acting enzyme, attacks the non-reducing end of the substrate. No activity is found on isomaltose, trehalose, sucrose, starch, amylose, glycogen, pullulan, xyloglucan, 4-nitrophenyl-alpha-D-galactoside, 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-mannoside, and 4-nitrophenyl-alpha-L-fucoside | Saccharolobus solfataricus | ? | - |
? | |
| additional information | exo-acting enzyme, attacks the non-reducing end of the substrate. No activity is found on isomaltose, trehalose, sucrose, starch, amylose, glycogen, pullulan, xyloglucan, 4-nitrophenyl-alpha-D-galactoside, 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-mannoside, and 4-nitrophenyl-alpha-L-fucoside | Saccharolobus solfataricus MT4 | ? | - |
? | |
| xyloglucan oligosaccharide + H2O | xyloglucan oligosaccharide from tamarind seed xyloglucan | Saccharolobus solfataricus | alpha-D-xylose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 72000, SDS-PAGE | Saccharolobus solfataricus |
| monomer | 1 * 84432, calculated from sequence | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-D-xyloside xylohydrolase | - |
Saccharolobus solfataricus |
| XylS | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
assay at | Saccharolobus solfataricus |
| 90 | - |
- |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
half-life: 38 h | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Saccharolobus solfataricus |
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