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Literature summary for 3.2.1.177 extracted from
- Structural and enzymatic characterisation of a glycoside hydrolase family 31 alpha-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification (2011), Biochem. J., 436, 567-580.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | saccharification of cell wall polymers | Cellvibrio japonicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Cellvibrio japonicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallographic structures of both the apo enzyme and the trapped covalent 5-fluoro-beta-xylosyl-enzyme intermediate, together with docking studies with the XXXG heptasaccharide the importance of a PA14 domain insert in the recognition of longer oligosaccharides by extension of the active-site pocket | Cellvibrio japonicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.39 | - |
4-nitrophenyl-alpha-D-xylopyranoside | pH 6, 25°C | Cellvibrio japonicus |  |
| 0.7 | - |
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc | pH 6, 25°C | Cellvibrio japonicus | |
| 55 | - |
Isoprimeverose | pH 6, 25°C | Cellvibrio japonicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| outer membrane | - |
Cellvibrio japonicus | 19867 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 113643 | - |
x * 113643, calculated from sequence | Cellvibrio japonicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellvibrio japonicus | B3PBD9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cellvibrio japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | 4-nitrophenol + alpha-D-xylose | - |
? | |
| isoprimeverose + H2O | kcat/KM is 11.5% compared to the value for 4-nitrophenyl-beta-isoprimeveroside | Cellvibrio japonicus | alpha-D-xylose + beta-D-glucose | - |
? | |
| additional information | CjXyl31A exhibits high regiospecificity for the hydrolysis of xylogluco-oligosaccharides, with a particular preference for longer substrates | Cellvibrio japonicus | ? | - |
? | |
| Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 113643, calculated from sequence | Cellvibrio japonicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xyl31A | - |
Cellvibrio japonicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Cellvibrio japonicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
4 h, 15% loss of activity | Cellvibrio japonicus |
| 45 | - |
4 h, 15% loss of activity | Cellvibrio japonicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
4-nitrophenyl-alpha-D-xylopyranoside | pH 6, 25°C | Cellvibrio japonicus | |
| 2.94 | - |
Isoprimeverose | pH 6, 25°C | Cellvibrio japonicus | |
| 9.39 | - |
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc | pH 6, 25°C | Cellvibrio japonicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Cellvibrio japonicus |
| 6.5 | - |
- |
Cellvibrio japonicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | pH 4.5: about 60% of maximal activity, pH 8.5: about 60% of maximal activity | Cellvibrio japonicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme is involved in xyloglucan saccharification | Cellvibrio japonicus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Isoprimeverose | pH 6, 25°C | Cellvibrio japonicus | |
| 1.48 | - |
4-nitrophenyl-alpha-D-xylopyranoside | pH 6, 25°C | Cellvibrio japonicus | |
| 13.4 | - |
Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc | pH 6, 25°C | Cellvibrio japonicus | |
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