Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by 2-mercaptoethanol | Thermothelomyces thermophilus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 25% (w/v) PEG 8K and 2.5 mM CaCl2 | Thermothelomyces thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 53% residual activity at 1 mM | Thermothelomyces thermophilus | |
ethanol | 41% residual activity at 1 mM | Thermothelomyces thermophilus | |
Fe2+ | 93% residual activity at 1 mM; % residual activity at 1 mM | Thermothelomyces thermophilus | |
methanol | 68% residual activity at 1 mM | Thermothelomyces thermophilus | |
SDS | complete inhibition at 0.1% (w/v) | Thermothelomyces thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.57 | - |
4-nitrophenyl beta-D-cellobioside | at 50°C in 50 mM sodium acetate (pH 5.0) | Thermothelomyces thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 107% activity at 1 mM | Thermothelomyces thermophilus | |
Co2+ | 127% activity at 1 mM | Thermothelomyces thermophilus | |
dithiothreitol | 108% activity at 1 mM | Thermothelomyces thermophilus | |
K+ | 115% activity at 1 mM | Thermothelomyces thermophilus | |
Li+ | 110% activity at 1 mM | Thermothelomyces thermophilus | |
Mg2+ | 115% activity at 1 mM | Thermothelomyces thermophilus | |
Mn2+ | 115% activity at 1 mM | Thermothelomyces thermophilus | |
additional information | not influenced by Ni2+ | Thermothelomyces thermophilus | |
Triton X-100 | 110% activity at 1% (v/v) | Thermothelomyces thermophilus | |
Tween 20 | 116% activity at 1% (v/v) | Thermothelomyces thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | Thermothelomyces thermophilus | - |
cellobiose + ? | - |
? | |
cellulose + H2O | Thermothelomyces thermophilus DSM 1799 | - |
cellobiose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces thermophilus | G2Q665 | - |
- |
Thermothelomyces thermophilus DSM 1799 | G2Q665 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thermothelomyces thermophilus |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography and DEAE-Sephadex gel filtration | Thermothelomyces thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | 0.9% activity compared to phosphoric acid-swollen cellulose | Thermothelomyces thermophilus | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | 0.9% activity compared to phosphoric acid-swollen cellulose | Thermothelomyces thermophilus DSM 1799 | 4-nitrophenol + cellobiose | - |
? | |
avicel + H2O | 4% activity compared to phosphoric acid-swollen cellulose | Thermothelomyces thermophilus | ? | - |
? | |
avicel + H2O | 4% activity compared to phosphoric acid-swollen cellulose | Thermothelomyces thermophilus DSM 1799 | ? | - |
? | |
bacterial cellulose + H2O | 13% activity compared to phosphoric acid-swollen cellulose | Thermothelomyces thermophilus | ? | - |
? | |
bacterial cellulose + H2O | 13% activity compared to phosphoric acid-swollen cellulose | Thermothelomyces thermophilus DSM 1799 | ? | - |
? | |
cellulose + H2O | - |
Thermothelomyces thermophilus | cellobiose + ? | - |
? | |
cellulose + H2O | - |
Thermothelomyces thermophilus DSM 1799 | cellobiose + ? | - |
? | |
filter paper + H2O | 21% activity compared to phosphoric acid-swollen cellulose | Thermothelomyces thermophilus | ? | - |
? | |
phosphoric acid-swollen cellulose + H2O | 100% activity | Thermothelomyces thermophilus | ? | - |
? | |
phosphoric acid-swollen cellulose + H2O | 100% activity | Thermothelomyces thermophilus DSM 1799 | ? | - |
? | |
sigmacell + H2O | 2% activity compared to phosphoric acid-swollen cellulose | Thermothelomyces thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Thermothelomyces thermophilus |
monomer | 1 * 53998, MALDI-TOF mass spectrometry | Thermothelomyces thermophilus |
monomer | 1 * 54000, calculated from amino acid sequence | Thermothelomyces thermophilus |
Synonyms | Comment | Organism |
---|---|---|
CBH | - |
Thermothelomyces thermophilus |
Cel7A | - |
Thermothelomyces thermophilus |
cellobiohydrolase | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermothelomyces thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | more than 45% activity between 50 and 75°C | Thermothelomyces thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the enzyme has a half-life of 60 min at 70°C and after incubation for 72 h retains more than 80% of enzymatic activity at 60°C and 100% at 50°C, respectively | Thermothelomyces thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.112 | - |
4-nitrophenyl beta-D-cellobioside | at 50°C in 50 mM sodium acetate (pH 5.0) | Thermothelomyces thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Thermothelomyces thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | more than 60% activity between pH 4.0 and 6.0 | Thermothelomyces thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.196 | - |
4-nitrophenyl beta-D-cellobioside | at 50°C in 50 mM sodium acetate (pH 5.0) | Thermothelomyces thermophilus |