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Literature summary for 3.2.1.176 extracted from

  • Kadowaki, M.A.S.; Higasi, P.; de Godoy, M.O.; Prade, R.A.; Polikarpov, I.
    Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila (2018), FEBS J., 285, 559-579 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information not influenced by 2-mercaptoethanol Thermothelomyces thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 25% (w/v) PEG 8K and 2.5 mM CaCl2 Thermothelomyces thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 53% residual activity at 1 mM Thermothelomyces thermophilus
ethanol 41% residual activity at 1 mM Thermothelomyces thermophilus
Fe2+ 93% residual activity at 1 mM; % residual activity at 1 mM Thermothelomyces thermophilus
methanol 68% residual activity at 1 mM Thermothelomyces thermophilus
SDS complete inhibition at 0.1% (w/v) Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
4-nitrophenyl beta-D-cellobioside at 50°C in 50 mM sodium acetate (pH 5.0) Thermothelomyces thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 107% activity at 1 mM Thermothelomyces thermophilus
Co2+ 127% activity at 1 mM Thermothelomyces thermophilus
dithiothreitol 108% activity at 1 mM Thermothelomyces thermophilus
K+ 115% activity at 1 mM Thermothelomyces thermophilus
Li+ 110% activity at 1 mM Thermothelomyces thermophilus
Mg2+ 115% activity at 1 mM Thermothelomyces thermophilus
Mn2+ 115% activity at 1 mM Thermothelomyces thermophilus
additional information not influenced by Ni2+ Thermothelomyces thermophilus
Triton X-100 110% activity at 1% (v/v) Thermothelomyces thermophilus
Tween 20 116% activity at 1% (v/v) Thermothelomyces thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Thermothelomyces thermophilus
-
cellobiose + ?
-
?
cellulose + H2O Thermothelomyces thermophilus DSM 1799
-
cellobiose + ?
-
?

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus G2Q665
-
-
Thermothelomyces thermophilus DSM 1799 G2Q665
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Thermothelomyces thermophilus

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography and DEAE-Sephadex gel filtration Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O 0.9% activity compared to phosphoric acid-swollen cellulose Thermothelomyces thermophilus 4-nitrophenol + cellobiose
-
?
4-nitrophenyl beta-D-cellobioside + H2O 0.9% activity compared to phosphoric acid-swollen cellulose Thermothelomyces thermophilus DSM 1799 4-nitrophenol + cellobiose
-
?
avicel + H2O 4% activity compared to phosphoric acid-swollen cellulose Thermothelomyces thermophilus ?
-
?
avicel + H2O 4% activity compared to phosphoric acid-swollen cellulose Thermothelomyces thermophilus DSM 1799 ?
-
?
bacterial cellulose + H2O 13% activity compared to phosphoric acid-swollen cellulose Thermothelomyces thermophilus ?
-
?
bacterial cellulose + H2O 13% activity compared to phosphoric acid-swollen cellulose Thermothelomyces thermophilus DSM 1799 ?
-
?
cellulose + H2O
-
Thermothelomyces thermophilus cellobiose + ?
-
?
cellulose + H2O
-
Thermothelomyces thermophilus DSM 1799 cellobiose + ?
-
?
filter paper + H2O 21% activity compared to phosphoric acid-swollen cellulose Thermothelomyces thermophilus ?
-
?
phosphoric acid-swollen cellulose + H2O 100% activity Thermothelomyces thermophilus ?
-
?
phosphoric acid-swollen cellulose + H2O 100% activity Thermothelomyces thermophilus DSM 1799 ?
-
?
sigmacell + H2O 2% activity compared to phosphoric acid-swollen cellulose Thermothelomyces thermophilus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Thermothelomyces thermophilus
monomer 1 * 53998, MALDI-TOF mass spectrometry Thermothelomyces thermophilus
monomer 1 * 54000, calculated from amino acid sequence Thermothelomyces thermophilus

Synonyms

Synonyms Comment Organism
CBH
-
Thermothelomyces thermophilus
Cel7A
-
Thermothelomyces thermophilus
cellobiohydrolase
-
Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermothelomyces thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 75 more than 45% activity between 50 and 75°C Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 the enzyme has a half-life of 60 min at 70°C and after incubation for 72 h retains more than 80% of enzymatic activity at 60°C and 100% at 50°C, respectively Thermothelomyces thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.112
-
4-nitrophenyl beta-D-cellobioside at 50°C in 50 mM sodium acetate (pH 5.0) Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Thermothelomyces thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
4 6 more than 60% activity between pH 4.0 and 6.0 Thermothelomyces thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.196
-
4-nitrophenyl beta-D-cellobioside at 50°C in 50 mM sodium acetate (pH 5.0) Thermothelomyces thermophilus