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Literature summary for 3.2.1.176 extracted from

  • Silva, J.C.; Guimaraes, L.H.; Salgado, J.C.; Furriel, R.P.; Polizeli, M.L.; Rosa, J.C.; Jorge, J.A.
    Purification and biochemical characterization of glucose-cellobiose-tolerant cellulases from Scytalidium thermophilum (2013), Folia Microbiol. (Praha), 58, 561-568 .
    View publication on PubMed
Search for more literature for 3.2.1.176

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, 177% of initial activity Mycothermus thermophilus
cysteine 1 mM, 132% of initial activity Mycothermus thermophilus
dithiothreitol 1 mM, 158% of initial activity Mycothermus thermophilus
Mn2+ 1 mM, 155% of initial activity Mycothermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 94% of initial activity Mycothermus thermophilus
Hg2+ 1 mM complete loss of activity Mycothermus thermophilus
NH4+ 1 mM, 70% of initial activity Mycothermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50400
-
 isoform cellulase I, gel filtration Mycothermus thermophilus

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 11% carbohydrate content Mycothermus thermophilus

Purification (Commentary)

Purification (Comment) Organism
-
 Mycothermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.7
-
 pH 4.0, 55°C Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
avicel + H2O
-
 Mycothermus thermophilus ?
-
 ?
carboxymethyl cellulose + H2O
-
 Mycothermus thermophilus cellobiose
-
 ?
additional information no substrates: starch, xylan, cellobiose. Filter paper is a very poor substrate Mycothermus thermophilus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 51200, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
 Mycothermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, stable Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Mycothermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Mycothermus thermophilus isoelectric focusing
-
 6.2