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Literature summary for 3.2.1.176 extracted from

  • Woon, J.; Mackeen, M.; Mahadi, N.; Illias, R.; Abdul Murad, A.; Abu Bakar, F.
    Expression and characterization of a cellobiohydrolase (CBH7B) from the thermophilic fungus Thielavia terrestris in Pichia pastoris (2016), Biotechnol. Appl. Biochem., 63, 690-698.
    View publication on PubMed
Search for more literature for 3.2.1.176

Application

Application Comment Organism
degradation enzyme works synergistically with the commercial enzyme cocktail Cellic R  CTec2 to enhance saccharification by 39% when added to a reaction mixture containing 0.25% alkaline pretreated oil palm empty fruit bunch Thermothielavioides terrestris

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermothielavioides terrestris

Inhibitors

Inhibitors Comment Organism Structure
cellobiose competitive Thermothielavioides terrestris
Cu2+ 1 and 10 mM, more than 70% inhibition Thermothielavioides terrestris
EDTA 1mM, 40% inhibition Thermothielavioides terrestris
Fe2+ 1 and 10 mM, more than 70% inhibition Thermothielavioides terrestris
sodium dodecylsulfate 1%, 53% inhibition Thermothielavioides terrestris
Urea 0.1 M, 55% inhibition Thermothielavioides terrestris
Zn2+ 1 and 10 mM, more than 70% inhibition Thermothielavioides terrestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 4-methylumbelliferyl beta-D-cellobioside pH 5.0, 55°C Thermothielavioides terrestris

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermothielavioides terrestris
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51800
-
 x * 51800, calculated Thermothielavioides terrestris

Organism

Organism UniProt Comment Textmining
Thermothielavioides terrestris G2QXZ9
-
-
Thermothielavioides terrestris ATCC 38088 G2QXZ9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Thermothielavioides terrestris 4-methylumbelliferol + cellobiose
-
 ?
4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Thermothielavioides terrestris ATCC 38088 4-methylumbelliferol + cellobiose
-
 ?
additional information no substrate: avicel Thermothielavioides terrestris ?
-
 ?
additional information no substrate: avicel Thermothielavioides terrestris ATCC 38088 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51800, calculated Thermothielavioides terrestris

Synonyms

Synonyms Comment Organism
Cbh7B
-
 Thermothielavioides terrestris
THITE_62596
-
 Thermothielavioides terrestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermothielavioides terrestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 24 h, 85% residual activtiy Thermothielavioides terrestris
60
-
 30 min, complete loss of activity Thermothielavioides terrestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 4-methylumbelliferyl beta-D-cellobioside pH 5.0, 55°C Thermothielavioides terrestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Thermothielavioides terrestris

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10
-
 Thermothielavioides terrestris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.185
-
 cellobiose pH 5.0, 55°C Thermothielavioides terrestris