Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.17 extracted from

  • Matano, M.; Nakajima, K.; Kashiwagi, Y.; Udaka, S.; Maehashi, K.
    Sweetness characterization of recombinant human lysozyme (2015), Comp. Biochem. Physiol. B, 188, 8-14 .
    View publication on PubMed
Search for more literature for 3.2.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Gallus gallus
expressed in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
D102G the mutant has 53% lytic activity and 47% chitinase activity compared to the wild type enzyme Homo sapiens
D53N the mutant shows complete loss of lytic and chitinase activities Homo sapiens
E35Q the mutant shows complete loss of lytic and chitinase activities Homo sapiens
W109Q the mutant has 55% lytic activity and 64% chitinase activity compared to the wild type enzyme Homo sapiens
Y63Q the mutant has 24% lytic activity and no chitinase activity compared to the wild type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidoglycan + H2O Gallus gallus
-
 ?
-
 ?
peptidoglycan + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus P00698
-
-
Homo sapiens P61626
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose column chromatography Gallus gallus
CM-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cell wall of Micrococcus luteus + H2O
-
 Gallus gallus ?
-
 ?
cell wall of Micrococcus luteus + H2O
-
 Homo sapiens ?
-
 ?
ethylene glycol chitin + H2O
-
 Gallus gallus ?
-
 ?
ethylene glycol chitin + H2O
-
 Homo sapiens ?
-
 ?
peptidoglycan + H2O
-
 Gallus gallus ?
-
 ?
peptidoglycan + H2O
-
 Homo sapiens ?
-
 ?

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
 9
Gallus gallus isoelectric focusing
-
 9.3

General Information

General Information Comment Organism
physiological function the enzyme activates the human sweet taste receptor T1R2/T1R3 Gallus gallus
physiological function the enzyme activates the human sweet taste receptor T1R2/T1R3 Homo sapiens