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Literature summary for 3.2.1.17 extracted from
- A single-step purification of cauliflower lysozyme and its dual role against bacterial and fungal plant pathogens (2015), Appl. Biochem. Biotechnol., 177, 556-566 .
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea | - |
cauliflower | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G100 gel filtration | Brassica oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, purified enzyme in sodium acetate buffer (50 mM, pH 5.6), 30 days, more than 90% loss of activity | Brassica oleracea |
| 37°C, purified enzyme in sodium acetate buffer (50 mM, pH 5.6), 30 days, complete loss of activity | Brassica oleracea |
| 4°C, purified enzyme in sodium acetate buffer (50 mM, pH 5.6), 30 days, 19% loss of activity | Brassica oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lyophilized cell walls of Micrococcus lysodiekticus + H2O | - |
Brassica oleracea | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22000, SDS-PAGE | Brassica oleracea |
| ? | x * 22000, calculated from amino acid sequence | Brassica oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Brassica oleracea |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | about 80% activity at 30°C, 100% activity at 40°C, about 40% activity at 50°C, about 15% activity at 60°C, no activity at 10, 20 or 70°C | Brassica oleracea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | the enzyme remains stable for 30 min at 10-40°C, activity sharply decreases to about 15% after 30 min at 50°C | Brassica oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Brassica oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | around 40-50% activity at pH 3.0-7.0, 100% activity at pH 8.0, about 20% activity at pH 9.0 | Brassica oleracea |
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Release 2023.1 (January 2023)
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