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Literature summary for 3.2.1.17 extracted from

  • Wang, N.; Wang, Y.; Li, G.; Sun, N.; Liu, D.
    Expression, characterization, and antimicrobial ability of T4 lysozyme from methylotrophic yeast Hansenula polymorpha A16 (2011), Sci. China Life Sci., 54, 520-526.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
T4 lysozyme is cloned into the yeast expression vector pPIC9K under the control of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase promoter and expressed at high level in Hansenula polymorpha strain A16, the enzyme is secreted Tequatrovirus T4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18700
-
1 * 18700 Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme from Hansenula polymorpha strain A16 culture supernatant by ammonium sulfate fractionation and ultrafiltration Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cell walls of Micrococcus lysodeikticus, are a substrate, while those of Xanthomonas campestris pv. malvacearum and Xanthomonas oryzae pv. oryzae are no substrates Tequatrovirus T4 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 18700 Tequatrovirus T4

Synonyms

Synonyms Comment Organism
T4 lysozyme
-
Tequatrovirus T4