Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.17 extracted from

  • Xue, Q.; Schey, K.L.; Volety, A.K.; Chu, F.E.; La Peyre, J.F.
    Purification and characterization of lysozyme from plasma of the eastern oyster (Crassostrea irginica) (2004), Comp. Biochem. Physiol. B, 139B, 11-25.
No PubMed abstract available
Search for more literature for 3.2.1.17

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 activates, maximal activity at 0.035 M Crassostrea virginica
MgCl2 activates, maximal activity at 0.035 M Crassostrea virginica
NaCl activity increases as the NaCl concentration is increased from 0 to 0.1 M and then decreases from its maximum activity at 0.1 M NaCl with further increase in NaCl concentrations Crassostrea virginica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17861
-
 x * 17861, MALDI-MS Crassostrea virginica
18400
-
 x * 18400, SDS-PAGE Crassostrea virginica

Organism

Organism UniProt Comment Textmining
Crassostrea virginica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Crassostrea virginica

Source Tissue

Source Tissue Comment Organism Textmining
hemolymph cell-free Crassostrea virginica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Crassostrea virginica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
peptidoglycan + H2O enzyme displays lytic activity against Lactococcus garvieae, Enterococcus sp., Vibrio vulnificus and Escherichia coli. The growth of Aeromonas hydrophila is inhibited only at a high concentration of 0.4 mg/ml. No growth inhibition of Streptococcus iniae and Aeromonas hydrophila Crassostrea virginica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 17771.5, two potential forms of enzyme: 17771.5 Da and 17861 Da, MALDI-MS Crassostrea virginica
? x * 17861, MALDI-MS Crassostrea virginica
? x * 18400, SDS-PAGE Crassostrea virginica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Crassostrea virginica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 10°C: about 60% of maximal activity, 60°C: about 60% of maximal activity Crassostrea virginica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Crassostrea virginica

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 60% of maximal activity within the range pH 5.0-7.5 Crassostrea virginica

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 13 30 min, stable Crassostrea virginica

pI Value

Organism Comment pI Value Maximum pI Value
Crassostrea virginica greater than 10
-
 additional information