Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold (DE3) pLyssS cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
D298N | inactive mutant which retains the ability to bind to O-GlcNAcylated peptides | Drosophila melanogaster |
D401A | the mutant displays 5fold increase in KM and 2400fold decrease in kcat compared to the wild type enzyme | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | Drosophila melanogaster | - |
[protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | Drosophila melanogaster | - |
[protein]-L-threonine + N-acetyl-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His Trap column chromatography | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | - |
Drosophila melanogaster | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
VPST(GlcNAc)MSAN + H2O | - |
Drosophila melanogaster | VPSTMSAN + N-acetyl-beta-D-glucosamine | - |
? | |
[host cell factor protein]-3-O-(N-acetyl-beta-D-hexosaminyl)-L-threonine + H2O | the sequence of host cell factor protein is IVPSVtASHSLR, t indicates the HexNAc-modified residue | Drosophila melanogaster | [host cell factor protein]-L-threonine + N-acetyl-D-hexosamine | - |
? | |
[nucleoporin 153]-3-O-(N-acetyl-beta-D-hexosaminyl)-L-serine + H2O | the seque4nce of nucleoporin 153 is FDLIIKPTVPVVsKPSTTDPIQSSK. s indicates the HexNAc-modified residue | Drosophila melanogaster | [nucleoporin 153]-L-serine + N-acetyl-D-hexosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | - |
Drosophila melanogaster | [protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | - |
Drosophila melanogaster | [protein]-L-threonine + N-acetyl-D-glucosamine | - |
? | |
[TAB1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | - |
Drosophila melanogaster | [TAB1 protein]-L-serine + N-acetyl-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAc hydrolase | - |
Drosophila melanogaster |
O-GlcNAcase | - |
Drosophila melanogaster |
OGA | - |
Drosophila melanogaster |