Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a drug target for the treatment of Alzheimer's and cardiovascular disease | Homo sapiens |
medicine | the enzyme is a drug target for the treatment of Alzheimer's and cardiovascular disease | Clostridium perfringens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-Gold cells | Clostridium perfringens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.175 M CdSO4 and 0.1 M sodium acetate at pH 7.5 | Clostridium perfringens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-(trifluoromethyl)-2,3-dihydro-1H-1,4-diazepine | - |
Clostridium perfringens | |
5-(trifluoromethyl)-2,3-dihydro-1H-1,4-diazepine | - |
Homo sapiens | |
GlcNAcstatin B | - |
Clostridium perfringens | |
GlcNAcstatin B | - |
Homo sapiens | |
GlcNAcstatin BF | - |
Clostridium perfringens | |
GlcNAcstatin BF | - |
Homo sapiens | |
GlcNAcstatin C | - |
Clostridium perfringens | |
GlcNAcstatin C | - |
Homo sapiens | |
GlcNAcstatin G | - |
Clostridium perfringens | |
GlcNAcstatin G | - |
Homo sapiens | |
thiamet G | - |
Clostridium perfringens | |
thiamet G | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | Homo sapiens | - |
[protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | Clostridium perfringens | - |
[protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | Homo sapiens | - |
[protein]-L-threonine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | Clostridium perfringens | - |
[protein]-L-threonine + N-acetyl-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | Q0TR53 | - |
- |
Homo sapiens | O60502 | - |
- |
Purification (Comment) | Organism |
---|---|
IMAC Sepharose column chromatography, Hi-Trap QFF column chromatography, and Superdex 200 gel filtration | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-N-acetylglucosaminide + H2O | - |
Homo sapiens | 4-methylumbelliferone + beta-D-N-acetylglucosamine | - |
? | |
4-methylumbelliferyl beta-D-N-acetylglucosaminide + H2O | - |
Clostridium perfringens | 4-methylumbelliferone + beta-D-N-acetylglucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | - |
Homo sapiens | [protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O | - |
Clostridium perfringens | [protein]-L-serine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | - |
Homo sapiens | [protein]-L-threonine + N-acetyl-D-glucosamine | - |
? | |
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O | - |
Clostridium perfringens | [protein]-L-threonine + N-acetyl-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAcase | - |
Homo sapiens |
O-GlcNAcase | - |
Clostridium perfringens |
OGA | - |
Homo sapiens |
OGA | - |
Clostridium perfringens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000163 | - |
GlcNAcstatin G | pH and temperature not specified in the publication | Clostridium perfringens | |
0.0000214 | - |
GlcNAcstatin G | pH and temperature not specified in the publication | Homo sapiens | |
0.0000232 | - |
GlcNAcstatin B | pH and temperature not specified in the publication | Homo sapiens | |
0.000037 | - |
thiamet G | pH and temperature not specified in the publication | Clostridium perfringens | |
0.000066 | - |
GlcNAcstatin B | pH and temperature not specified in the publication | Clostridium perfringens | |
0.0001531 | - |
thiamet G | pH and temperature not specified in the publication | Homo sapiens | |
0.146 | - |
5-(trifluoromethyl)-2,3-dihydro-1H-1,4-diazepine | pH and temperature not specified in the publication | Clostridium perfringens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000145 | - |
pH and temperature not specified in the publication | Clostridium perfringens | GlcNAcstatin B | |
0.0000429 | - |
pH and temperature not specified in the publication | Clostridium perfringens | GlcNAcstatin G | |
0.0000578 | - |
pH and temperature not specified in the publication | Homo sapiens | GlcNAcstatin G | |
0.0000626 | - |
pH and temperature not specified in the publication | Clostridium perfringens | thiamet G | |
0.0000632 | - |
pH and temperature not specified in the publication | Homo sapiens | GlcNAcstatin B | |
0.0001501 | - |
pH and temperature not specified in the publication | Homo sapiens | thiamet G |