Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D174A | generated mutant, reveals severely impaired catalytic activity across a broad range of substrates alongside a pH-activity profile consistent with deletion of a key catalytic residue | Homo sapiens |
D175A | generated mutant, significant decrease in catalytic efficiency with substrates bearing poor leaving groups (up to 3000fold), while for substrates bearing good leading groups the difference is much smaller (7fold). Mutant enzyme cleaves thioglycosides with essentially the same catalytic efficiency as the wild-type enzyme | Homo sapiens |
DELTA1-350 | deletion construct comprising amino acids 1-350 of human O-GlcNAcase | Homo sapiens |
DELTA351-916 | deletion construct comprising amino acids 351-916 of human O-GlcNAcase | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | wild-type | Homo sapiens | |
0.2 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D175A mutant | Homo sapiens | |
0.21 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild-type | Homo sapiens | |
0.21 | - |
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide | wild-type | Homo sapiens | |
0.22 | - |
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide | D175A mutant | Homo sapiens | |
0.23 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D175A mutant | Homo sapiens | |
0.3 | - |
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide | wild-type | Homo sapiens | |
0.31 | - |
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide | D175A mutant | Homo sapiens | |
0.36 | - |
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide | D174A mutant (pH 5.0) | Homo sapiens | |
0.38 | - |
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide | D174A mutant (pH 7.4) | Homo sapiens | |
0.46 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D174A mutant (pH 5.0) | Homo sapiens | |
0.47 | - |
3-nitrophenyl N-acetyl-beta-D-glucosaminide | wild-type | Homo sapiens | |
0.56 | - |
4-chlorophenyl N-acetyl-beta-D-glucosaminide | wild-type | Homo sapiens | |
0.56 | - |
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide | D174A mutant (pH 7.4) | Homo sapiens | |
0.62 | - |
phenyl N-acetyl-beta-D-glucosaminide | wild-type | Homo sapiens | |
0.73 | - |
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide | D174A mutant (pH 5.0) | Homo sapiens | |
0.84 | - |
N-acetyl-4-aminophenyl N-acetyl-beta-D-glucosaminide | wild-type | Homo sapiens | |
0.95 | - |
3-nitrophenyl N-acetyl-beta-D-glucosaminide | D175A mutant | Homo sapiens | |
0.95 | - |
phenyl N-acetyl-beta-D-glucosaminide | D175A mutant | Homo sapiens | |
1.4 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | D174A mutant (pH 7.4) | Homo sapiens | |
1.4 | - |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide | D174A mutant (pH 7.4) | Homo sapiens | |
1.5 | - |
4-nitrophenyl N-acetyl-beta-D-glucosaminide | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | catalyzing the hydrolytic cleavage of beta-O-linked 2-acetamido-2-deoxy-D-glycopyranose (O-GlcNAc) from serine and threonine residues of posttranslationally modified proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-affinity chromotography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 3,4-dinitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 3-fluoro-4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
3-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 3-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-chlorophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-chlorophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-methylumbelliferone + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
additional information | catalyzing the hydrolytic cleavage of beta-O-linked 2-acetamido-2-deoxy-D-glycopyranose (O-GlcNAc) from serine and threonine residues of posttranslationally modified proteins | Homo sapiens | ? | - |
? | |
N-acetyl-4-aminophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | N-acetyl-4-aminophenol + N-acetyl-beta-D-glucosamine | - |
? | |
phenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Homo sapiens | phenol + N-acetyl-beta-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O-GlcNAcase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
measured pH-values range from 4.5 to 9.0, hydrolysis of 4-nitrophenyl N-acetyl-beta-D-glucosaminide resembles a bell-shaped curve with the maximal catalytic efficiency at a pH of 6.5 | Homo sapiens |
7.4 | - |
assay at | Homo sapiens |