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Literature summary for 3.2.1.166 extracted from
- 1H NMR spectroscopic studies establish that heparanase is a retaining glycosidase (2014), Biochem. Biophys. Res. Commun., 443, 185-188.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | heparanase is an important target for anti-cancer and anti-inflammatory drug discovery | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the enzyme in Spodoptera frugiperda Sf9 cells and secretion to the cell culture medium | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | heparanase is an endo-beta-glucuronidase that cleaves heparan sulfate side chains of proteoglycans in basement membranes and the extracellular matrix | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fondaparinux + H2O | a pentasaccharide substrate, heparanase is a retaining glycosidase, NMR spectroscopic analysis, overview | Homo sapiens | 2N-sulfo-6-O-sulfo-alpha-D-GlcN-(1->4)-beta-GlcA + a trisaccharide | - |
? | |
| additional information | heparanase is an endo-beta-glucuronidase that cleaves heparan sulfate side chains of proteoglycans in basement membranes and the extracellular matrix | Homo sapiens | ? | - |
? | |
| additional information | the enzyme hydrolyses the glycosidic bond between the (1->4)-alpha-GlcA and the 2-N-sulfo-3,6-di-O-sulfo-(1->4)-alpha-D-GlcN | Homo sapiens | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the clan A glycoside hydrolase family 79, GH79 | Homo sapiens |
| physiological function | the enzyme is implicated in several diverse pathological processes associated with extracellular matrix degradation such as metastasis, inflammation and angiogenesis | Homo sapiens |
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Release 2023.1 (January 2023)
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