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Literature summary for 3.2.1.166 extracted from

  • Bame, K.J.
    Heparanases: endoglycosidases that degrade heparan sulfate proteoglycans (2001), Glycobiology, 11, 91R-98R.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
basic fibroblast growth factor
-
Cricetulus griseus
additional information no inhibition by basic fibroblast growth factor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
heparan sulfate proteoglycan + H2O Homo sapiens heparanase cleaves the heparan sulfate glycosaminoglycans from proteoglycan core proteins and degrades them to small oligosaccharides. Inside cells, the enzyme is important for the normal catabolism of heparan sulfate proteoglycans, generating glycosaminoglycan fragments that are then transported to lysosomes and completely degraded. When secreted, heparanase degrades basement membrane heparan sulfate glycosaminoglycans at sites of injury or inflammation, allowing extravasion of immune cells into nonvascular spaces and releasing factors that regulate cell proliferation and angiogenesis. At physiological pH the enzyme binds to the extracellular matrix of cell surface heparan sulfate proteolycan but is inactive. When the pH is lowered, which could occur at sites of inflammation or matrix damage, the bound enzyme becomes active and cleaves the heparan sulfate proteoglycan it is bound to heparan sulfate fragment + truncated heparan sulfate proteoglycan
-
?
heparan sulfate proteolycan + H2O Cricetulus griseus
-
heparan sulfate fragment + truncated heparan sulfate proteoglycan
-
?

Organism

Organism UniProt Comment Textmining
Cricetulus griseus
-
-
-
Homo sapiens Q9Y251
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein synthesized as an inactive 65000 Da glycoprotein that is cleaved at the N-terminus to generate the active enzyme Cricetulus griseus
glycoprotein synthesized as an inactive 65000 Da glycoprotein that is cleaved at the N-terminus to generate the active enzyme Homo sapiens
proteolytic modification synthesized as an inactive 65000 Da glycoprotein that is cleaved at the N-terminus to generate the active enzyme Cricetulus griseus
proteolytic modification synthesized as an inactive 65000 Da glycoprotein that is cleaved at the N-terminus to generate the active enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet
-
Homo sapiens
-
CHO cell
-
Cricetulus griseus
-
additional information highly expressed in tumors and metastatic cell lines derived from a variety of tissues Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparan sulfate proteoglycan + H2O heparanase cleaves the heparan sulfate glycosaminoglycans from proteoglycan core proteins and degrades them to small oligosaccharides. Inside cells, the enzyme is important for the normal catabolism of heparan sulfate proteoglycans, generating glycosaminoglycan fragments that are then transported to lysosomes and completely degraded. When secreted, heparanase degrades basement membrane heparan sulfate glycosaminoglycans at sites of injury or inflammation, allowing extravasion of immune cells into nonvascular spaces and releasing factors that regulate cell proliferation and angiogenesis. At physiological pH the enzyme binds to the extracellular matrix of cell surface heparan sulfate proteolycan but is inactive. When the pH is lowered, which could occur at sites of inflammation or matrix damage, the bound enzyme becomes active and cleaves the heparan sulfate proteoglycan it is bound to Homo sapiens heparan sulfate fragment + truncated heparan sulfate proteoglycan
-
?
heparan sulfate proteoglycan + H2O cleavage of the beta-1,4-glycosidic bond between a D-glucuronate and a D-glucosamine in heparan sulfate. Cleavage to short chains of 6000-8000 Da. 2-O-sulfate groups are not required for C1A heparanase to recognize and degrade the substrate Cricetulus griseus heparan sulfate fragment + truncated heparan sulfate proteoglycan
-
?
heparan sulfate proteoglycan + H2O cleavage to 5000 Da oligosaccharides Homo sapiens heparan sulfate fragment + truncated heparan sulfate proteoglycan
-
?
heparan sulfate proteolycan + H2O
-
Cricetulus griseus heparan sulfate fragment + truncated heparan sulfate proteoglycan
-
?
heparin sulfate + H2O cleavage to 5000 Da oligosaccharides Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
C1A heparanase
-
Cricetulus griseus
Hpa1 heparanase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 5.8
-
Cricetulus griseus
5.5 5.8
-
Homo sapiens