Inhibitors | Comment | Organism | Structure |
---|---|---|---|
basic fibroblast growth factor | - |
Cricetulus griseus | |
additional information | no inhibition by basic fibroblast growth factor | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparan sulfate proteoglycan + H2O | Homo sapiens | heparanase cleaves the heparan sulfate glycosaminoglycans from proteoglycan core proteins and degrades them to small oligosaccharides. Inside cells, the enzyme is important for the normal catabolism of heparan sulfate proteoglycans, generating glycosaminoglycan fragments that are then transported to lysosomes and completely degraded. When secreted, heparanase degrades basement membrane heparan sulfate glycosaminoglycans at sites of injury or inflammation, allowing extravasion of immune cells into nonvascular spaces and releasing factors that regulate cell proliferation and angiogenesis. At physiological pH the enzyme binds to the extracellular matrix of cell surface heparan sulfate proteolycan but is inactive. When the pH is lowered, which could occur at sites of inflammation or matrix damage, the bound enzyme becomes active and cleaves the heparan sulfate proteoglycan it is bound to | heparan sulfate fragment + truncated heparan sulfate proteoglycan | - |
? | |
heparan sulfate proteolycan + H2O | Cricetulus griseus | - |
heparan sulfate fragment + truncated heparan sulfate proteoglycan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
- |
- |
Homo sapiens | Q9Y251 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | synthesized as an inactive 65000 Da glycoprotein that is cleaved at the N-terminus to generate the active enzyme | Cricetulus griseus |
glycoprotein | synthesized as an inactive 65000 Da glycoprotein that is cleaved at the N-terminus to generate the active enzyme | Homo sapiens |
proteolytic modification | synthesized as an inactive 65000 Da glycoprotein that is cleaved at the N-terminus to generate the active enzyme | Cricetulus griseus |
proteolytic modification | synthesized as an inactive 65000 Da glycoprotein that is cleaved at the N-terminus to generate the active enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood platelet | - |
Homo sapiens | - |
CHO cell | - |
Cricetulus griseus | - |
additional information | highly expressed in tumors and metastatic cell lines derived from a variety of tissues | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heparan sulfate proteoglycan + H2O | heparanase cleaves the heparan sulfate glycosaminoglycans from proteoglycan core proteins and degrades them to small oligosaccharides. Inside cells, the enzyme is important for the normal catabolism of heparan sulfate proteoglycans, generating glycosaminoglycan fragments that are then transported to lysosomes and completely degraded. When secreted, heparanase degrades basement membrane heparan sulfate glycosaminoglycans at sites of injury or inflammation, allowing extravasion of immune cells into nonvascular spaces and releasing factors that regulate cell proliferation and angiogenesis. At physiological pH the enzyme binds to the extracellular matrix of cell surface heparan sulfate proteolycan but is inactive. When the pH is lowered, which could occur at sites of inflammation or matrix damage, the bound enzyme becomes active and cleaves the heparan sulfate proteoglycan it is bound to | Homo sapiens | heparan sulfate fragment + truncated heparan sulfate proteoglycan | - |
? | |
heparan sulfate proteoglycan + H2O | cleavage of the beta-1,4-glycosidic bond between a D-glucuronate and a D-glucosamine in heparan sulfate. Cleavage to short chains of 6000-8000 Da. 2-O-sulfate groups are not required for C1A heparanase to recognize and degrade the substrate | Cricetulus griseus | heparan sulfate fragment + truncated heparan sulfate proteoglycan | - |
? | |
heparan sulfate proteoglycan + H2O | cleavage to 5000 Da oligosaccharides | Homo sapiens | heparan sulfate fragment + truncated heparan sulfate proteoglycan | - |
? | |
heparan sulfate proteolycan + H2O | - |
Cricetulus griseus | heparan sulfate fragment + truncated heparan sulfate proteoglycan | - |
? | |
heparin sulfate + H2O | cleavage to 5000 Da oligosaccharides | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C1A heparanase | - |
Cricetulus griseus |
Hpa1 heparanase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 5.8 | - |
Cricetulus griseus |
5.5 | 5.8 | - |
Homo sapiens |