Literature summary for 3.2.1.166 extracted from

McKenzie, E.; Young, K.; Hircock, M.; Bennett, J.; Bhaman, M.; Felix, R.; Turner, P.; Stamps, A.; McMillan, D.; Saville, G.; Ng, S.; Mason, S.; Snell, D.; Schofield, D.; Gong, H.; Townsend, R.; Gallagher, J.; Page, M.; Parekh, R.; Stubberfield, C.
Biochemical characterization of the active heterodimer form of human heparanase (Hpa1) protein expressed in insect cells (2003), Biochem. J., 373, 423-435.

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Cloned(Commentary)

Cloned (Comment)	Organism
insect cell expression system is used for the generation of large amounts of recombinant protein of high specific activity. Individual subunits are cloned into baculoviral secretory vectors and coexpressed in insect cells	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9Y251	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	glycosylation of Hpa1 is essential for solubility	Homo sapiens
proteolytic modification	Hpa1 protein is initially synthesized as an inactive 65000 Da proenzyme and subsequently activated by proteolytic cleavage to generate an active heterodimer of 8000 Da and 50000 Da polypeptides	Homo sapiens

Storage Stability

Storage Stability	Organism
4°C, loss of 50% activity after storage for 1 year, recombinantly expressed enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
heparan sulphate proteoglycan + H2O	-	Homo sapiens	heparan sulfate fragment + truncated heparan sulfate proteoglycan	-	?

Subunits

Subunits	Comment	Organism
heterodimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Hpa1	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	5.5	pH 4.0: about 80% of maximal activity, pH 5.5: about 60% of maximal activity. pH 7.0: about 20% of maximal activity	Homo sapiens

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Release 2023.1 (January 2023)