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Literature summary for 3.2.1.165 extracted from

  • Aktuganov, G.E.; Galimzianova, N.F.; Gilvanova, E.A.; Pudova, E.A.; Kuzmina, L.Y.; Melentiev, A.I.; Safina, V.R.
    Purification and characterization of exo-beta-1,4-glucosaminidase produced by chitosan-degrading fungus, Penicillium sp. IB-37-2A (2019), World J. Microbiol. Biotechnol., 35, 18 .
    View publication on PubMed
Search for more literature for 3.2.1.165

Activating Compound

Activating Compound Comment Organism Structure
PEG 3000 105.4% activity at 1 mM Penicillium sp. IB-37-2A
Triton X-100 110% activity at 1 mM Penicillium sp. IB-37-2A
Tween-80 115% activity at 10 mM Penicillium sp. IB-37-2A
Urea 112% activity at 1 mM Penicillium sp. IB-37-2A

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Penicillium sp. IB-37-2A
Cu2+ complete inhibition at 10 mM Penicillium sp. IB-37-2A
Fe2+ 82.5% residual activity at 10 mM Penicillium sp. IB-37-2A
Hg2+ complete inhibition at 1 mM Penicillium sp. IB-37-2A
Ni2+ 40.5% residual activity at 10 mM Penicillium sp. IB-37-2A
SDS 54% residual activity at 10 mM Penicillium sp. IB-37-2A
Urea 90.4% residual activity at 10 mM Penicillium sp. IB-37-2A
Zn2+ 36.5% residual activity at 10 mM Penicillium sp. IB-37-2A

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 124% activity at 10 mM Penicillium sp. IB-37-2A
Mg2+ 123.3% activity at 1 mM Penicillium sp. IB-37-2A

Organism

Organism UniProt Comment Textmining
Penicillium sp. IB-37-2A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, affinity sorption on colloidal chitosan and hydrophobic chromatography on phenyl-Sepharose CL 4B Penicillium sp. IB-37-2A

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.121
-
 culture filtrate, at pH 4.0 and 50°C Penicillium sp. IB-37-2A
5.643
-
 after 46.6fold purification, at pH 4.0 and 50°C Penicillium sp. IB-37-2A

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Penicillium sp. IB-37-2A ?
-
 ?
beta-glucan + H2O 13.6% activity compared to soluble chitosan Penicillium sp. IB-37-2A ?
-
 ?
colloidal chitin + H2O deacetylation degree 80-85%, 6.4% activity compared to soluble chitosan Penicillium sp. IB-37-2A D-glucosamine + ?
-
 ?
glycol chitosan + H2O 631.3% activity compared to soluble chitosan Penicillium sp. IB-37-2A D-glucosamine + ?
-
 ?
laminarin + H2O 21.5% activity compared to soluble chitosan Penicillium sp. IB-37-2A ?
-
 ?
additional information no activity with carboxymethyl cellulose, cellulose, xylan and galactomannan Penicillium sp. IB-37-2A ?
-
 ?
soluble chitosan + H2O deacetylation degree 80-85%, 100% activity Penicillium sp. IB-37-2A D-glucosamine + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Penicillium sp. IB-37-2A

Synonyms

Synonyms Comment Organism
beta-GlcNase
-
 Penicillium sp. IB-37-2A
exo-beta-1.4-glucosaminidase
-
 Penicillium sp. IB-37-2A
exo-chitosanase
-
 Penicillium sp. IB-37-2A

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
 Penicillium sp. IB-37-2A

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the enzyme demonstrates high temperature stability at 30-55°C and is almost inactive at 65°C Penicillium sp. IB-37-2A

pH Range

pH Minimum pH Maximum Comment Organism
4
-
-
 Penicillium sp. IB-37-2A

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 the enzyme demonstrates high pH stability at pH 3.0-8.0, and it loses more than 50% of its original activity at pH values above 8.0 Penicillium sp. IB-37-2A

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium sp. IB-37-2A isoelectric focusing
-
 4.9