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Literature summary for 3.2.1.165 extracted from
- Enzymatic production of glucosamine and chitooligosaccharides using newly isolated exo-beta-D-glucosaminidase having transglycosylation activity (2016), 3 Biotech, 6, 13 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 120% activity at 1 mM | Aspergillus fumigatus |  |
| NaCl | the optimum NaCl concentration for the enzyme is 250 mM | Aspergillus fumigatus | |
General Stability
| General Stability | Organism |
|---|---|
| electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme retains more than 50% of its catalytic activity after 10 batches of uses in aqueous solution | Aspergillus fumigatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Hydroxy-5-nitrobenzyl bromide | 65% residual activity at 1 mM | Aspergillus fumigatus | |
| 4-chloromercuribenzoate | 88% residual activity at 1 mM | Aspergillus fumigatus | |
| Ba2+ | 98% residual activity at 1 mM | Aspergillus fumigatus | |
| Ca2+ | 88% residual activity at 1 mM | Aspergillus fumigatus | |
| Cd2+ | 90% residual activity at 1 mM | Aspergillus fumigatus | |
| Co2+ | 32% residual activity at 1 mM | Aspergillus fumigatus | |
| Cu2+ | 98% residual activity at 1 mM | Aspergillus fumigatus | |
| dithiothreitol | 90% residual activity at 1 mM | Aspergillus fumigatus | |
| Fe2+ | 87% residual activity at 1 mM | Aspergillus fumigatus | |
| Hg2+ | 76% residual activity at 1 mM | Aspergillus fumigatus | |
| iodoacetate | 93% residual activity at 1 mM | Aspergillus fumigatus | |
| Mg2+ | 95% residual activity at 1 mM | Aspergillus fumigatus | |
| Mn2+ | 98% residual activity at 1 mM | Aspergillus fumigatus | |
| N-ethylmaleimide | 66% residual activity at 1 mM | Aspergillus fumigatus | |
| Ni2+ | 87% residual activity at 1 mM | Aspergillus fumigatus | |
| Triton X-100 | 88% residual activity at 1 mM | Aspergillus fumigatus | |
| Tween 20 | 76% residual activity at 1 mM | Aspergillus fumigatus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 102% activity at 1 mM | Aspergillus fumigatus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | Aspergillus fumigatus | - |
D-glucosamine + ? | - |
? | |
| chitosan + H2O | Aspergillus fumigatus IIT-004 | - |
D-glucosamine + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
| Aspergillus fumigatus IIT-004 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone fractionation, DEAE Sepharose column chromatography, CM-Sepharose column chromatography, and Superdex 200 gel filtration | Aspergillus fumigatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus fumigatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.5 | - |
crude enzyme, at pH 5.5 and 40°C | Aspergillus fumigatus |
| 13 | - |
after 26fold purification, at pH 5.5 and 40°C | Aspergillus fumigatus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme, 30 days, 40% loss of activity | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
| chitin + H2O | - |
Aspergillus fumigatus IIT-004 | ? | - |
? | |
| chitosan + H2O | - |
Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
| chitosan + H2O | chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
| chitosan + H2O | chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
| chitosan + H2O | chitosan with more than 70% degree of acetylation, 43% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
| chitosan + H2O | chitosan with more than 80% degree of acetylation, 68% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
| chitosan + H2O | - |
Aspergillus fumigatus IIT-004 | D-glucosamine + ? | - |
? | |
| chitosan + H2O | chitosan with more than 50% degree of acetylation, 9% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation | Aspergillus fumigatus IIT-004 | D-glucosamine + ? | - |
? | |
| chitosan + H2O | chitosan with more than 60% degree of acetylation, 21% activity compared to crab shell chitosan chitosan with more than 90% degree of acetylation | Aspergillus fumigatus IIT-004 | D-glucosamine + ? | - |
? | |
| colloidal chitosan + H2O | 56% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
| crab shell chitosan + H2O | more than 90% degree of acetylation, 100% activity | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
| D-glucosamine + N-acetyl-D-glucosamine | 15% (w/v) ammonium sulfate is optimum for catalyzing transglycosylation reaction | Aspergillus fumigatus | chitodimer | - |
? | |
| glycol chitosan + H2O | 12% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
| additional information | no activity with flaked chitin, colloidal chitin and cellulose | Aspergillus fumigatus | ? | - |
? | |
| additional information | no activity with flaked chitin, colloidal chitin and cellulose | Aspergillus fumigatus IIT-004 | ? | - |
? | |
| shrimp shell chitosan + H2O | more than 90% degree of acetylation, 57% activity compared to crab shell chitosan with more than 90% degree of acetylation | Aspergillus fumigatus | D-glucosamine + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, SDS-PAGE | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-beta-D-glucosaminidase | - |
Aspergillus fumigatus |
| exochitosanase | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
free enzyme | Aspergillus fumigatus |
| 50 | - |
electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme | Aspergillus fumigatus |
| 50 | - |
optimum for transglycosylation reaction | Aspergillus fumigatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the enzyme remains stable at up to 50°C for 32 h | Aspergillus fumigatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3000 | - |
crab shell chitosan | at pH 5.5 and 40°C | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
free enzyme | Aspergillus fumigatus |
| 5.8 | - |
electrospun polyacrylonitrile nanofibrous membranes-immobilized enzyme | Aspergillus fumigatus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus fumigatus | isoelectric focusing | - |
6.5 |
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