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Literature summary for 3.2.1.165 extracted from

  • Jung, W.J.; Kuk, J.H.; Kim, K.Y.; Jung, K.C.; Park, R.D.
    Purification and characterization of exo-beta-D-glucosaminidase from Aspergillus fumigatus S-26 (2006), Protein Expr. Purif., 45, 125-131.
    View publication on PubMed
Search for more literature for 3.2.1.165

Inhibitors

Inhibitors Comment Organism Structure
2-Hydroxy-5-nitrobenzyl bromide 2.5 mM, complete inhibition Aspergillus fumigatus
AlCl3 10 mM, 37% inhibition Aspergillus fumigatus
CuSO4 10 mM, 55% inhibition Aspergillus fumigatus
HgCl2 10 mM, complete inhibition Aspergillus fumigatus
N-bromosuccinimide 10 mM, 34% inhibition Aspergillus fumigatus
p-chloromercuribenzoic acid 2.5 mM, 86% inhibition Aspergillus fumigatus
Pb(NO3)2 10 mM, 47% inhibition Aspergillus fumigatus
ZnSO4 10 mM, 21% inhibition Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-value for chitosan is 1.0 mg/ml Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus fumigatus
-
-

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus S-26
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus fumigatus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.19
-
-
 Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitobiose + H2O
-
 Aspergillus fumigatus D-glucosamine
-
 ?
chitobiose + H2O
-
 Aspergillus fumigatus S-26 D-glucosamine
-
 ?
chitoheptaose + H2O products at an early stage of reaction Aspergillus fumigatus D-glucosamine + chitohexaose
-
 ?
chitoheptaose + H2O products at an early stage of reaction Aspergillus fumigatus S-26 D-glucosamine + chitohexaose
-
 ?
chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine Aspergillus fumigatus D-glucosamine + chitopentaose
-
 ?
chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine Aspergillus fumigatus S-26 D-glucosamine + chitopentaose
-
 ?
chitopentaose + H2O products at an early stage of reaction Aspergillus fumigatus D-glucosamine + chitotetraose
-
 ?
chitopentaose + H2O products at an early stage of reaction Aspergillus fumigatus S-26 D-glucosamine + chitotetraose
-
 ?
chitosan + H2O fully deacetylated chitosan Aspergillus fumigatus D-glucosamine + ?
-
 ?
chitotetraose + H2O products at an early stage of reaction Aspergillus fumigatus D-glucosamine + chitotriose
-
 ?
chitotriose + H2O products at an early stage of reaction Aspergillus fumigatus D-glucosamine + chitobiose
-
 ?
additional information no degradation of cellulose and starch. The exo-beta-D-glucosaminidase exhibits transglycosylation activity, resulting in the one-residue elongated oligomers Aspergillus fumigatus ?
-
 ?
additional information no degradation of cellulose and starch. The exo-beta-D-glucosaminidase exhibits transglycosylation activity, resulting in the one-residue elongated oligomers Aspergillus fumigatus S-26 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 Aspergillus fumigatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 40% of maximal activity, 70°C: about 75% of maximal activity Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 stable below Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28.3
-
 chitosan
-
 Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 6
-
 Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 10 stable Aspergillus fumigatus