Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.164 extracted from

  • Sakamoto, T.; Taniguchi, Y.; Suzuki, S.; Ihara, H.; Kawasaki, H.
    Characterization of Fusarium oxysporum beta-1,6-galactanase, an enzyme that hydrolyzes larch wood arabinogalactan (2007), Appl. Environ. Microbiol., 73, 3109-3112.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.164

Cloned(Commentary)

Cloned (Comment) Organism
gene Fogal1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha as maltose-binding-protein fusion enzyme Fusarium oxysporum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 50% inhibition Fusarium oxysporum
Cd2+ 1 mM, 45% inhibition Fusarium oxysporum
Cu2+ 1 mM, 45% inhibition Fusarium oxysporum
Fe2+ 1 mM, 65% inhibition Fusarium oxysporum
Hg2+ 1 mM, 80% inhibition Fusarium oxysporum
additional information no inhibition by EDTA Fusarium oxysporum
Zn2+ 1 mM, 60% inhibition Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Fusarium oxysporum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme activity does not require metal ions and is not affected by the presence of Ba2+, Ca2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, or Ni2+, overview Fusarium oxysporum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 47000, SDS-PAGE Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
 gene Fogal1
-
Fusarium oxysporum 12S
-
 gene Fogal1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding-protein fusion enzyme from Escherichia coli strain DH5alpha, by cation exchange and amylose affinity chromatography, followed by hydrophobic interaction chromatography, native enzyme 88fold from strain S12 culture filtrate by anion exchange and hydrophobic interaction chromatography, followed by cation exchange chromatography, dialysis and gel filtration Fusarium oxysporum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.55
-
 purified native enzyme Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
type II arabinogalactans + H2O the enzyme preferentially debranches beta-1,6-galactobiose from the substrate, type II arabinogalactans from Western larch Fusarium oxysporum ?
-
 ?
type II arabinogalactans + H2O the enzyme preferentially debranches beta-1,6-galactobiose from the substrate, type II arabinogalactans from Western larch Fusarium oxysporum 12S ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Fusarium oxysporum

Synonyms

Synonyms Comment Organism
beta-1,6-galactanase
-
 Fusarium oxysporum
GAL1
-
 Fusarium oxysporum
More the enzyme belongs to the glycoside hydrolase family 5 Fusarium oxysporum
type II arabinogalactan-degrading enzyme
-
 Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Fusarium oxysporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, purified native enzyme, over 80% remaining activity Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Fusarium oxysporum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 16 h, purified native enzyme, over 80% remaining activity Fusarium oxysporum