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Literature summary for 3.2.1.158 extracted from

  • Zhang, W.; Xu, J.; Liu, D.; Liu, H.; Lu, X.; Yu, W.
    Characterization of an alpha-agarase from Thalassomonas sp. LD5 and its hydrolysate (2018), Appl. Microbiol. Biotechnol., 102, 2203-2212 .
    View publication on PubMed
Search for more literature for 3.2.1.158

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli AD494(DE3) cells Thalassomonas sp. LD5

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 3% residual activity at 2 mM Thalassomonas sp. LD5
Ba2+ 90% residual activity at 2 mM Thalassomonas sp. LD5
Cu2+ 5% residual activity at 2 mM Thalassomonas sp. LD5
EDTA 1% residual activity at 2 mM Thalassomonas sp. LD5
Fe2+ 18% residual activity at 2 mM Thalassomonas sp. LD5
Fe3+ complete inhibition at 2 mM Thalassomonas sp. LD5
Mg2+ 70% residual activity at 2 mM Thalassomonas sp. LD5
Mn2+ complete inhibition at 2 mM Thalassomonas sp. LD5
NH4+ 84% residual activity at 2 mM Thalassomonas sp. LD5
Ni2+ 28% residual activity at 2 mM Thalassomonas sp. LD5
SDS 39% residual activity at 2 mM Thalassomonas sp. LD5
Zn2+ 10% residual activity at 2 mM Thalassomonas sp. LD5

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme is strongly dependent on the presence of Ca2+ ions (170% activity at 10 mM) Thalassomonas sp. LD5
Li+ 105% activity at 2 mM Thalassomonas sp. LD5
Na+ 107% activity at 2 mM Thalassomonas sp. LD5

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
360000
-
 gel filtration Thalassomonas sp. LD5

Organism

Organism UniProt Comment Textmining
Thalassomonas sp. LD5 A0A1S5VH75
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-Sepharose column chromatography and Superdex S200 gel filtration Thalassomonas sp. LD5

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agar + H2O
-
 Thalassomonas sp. LD5 agarotetraose
-
 ?
agarotetraose + H2O
-
 Thalassomonas sp. LD5 neoagarotriose
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 180000, SDS-PAGE Thalassomonas sp. LD5
homodimer 2 * 154000, calculated from amino acid sequence Thalassomonas sp. LD5

Synonyms

Synonyms Comment Organism
AgaD
-
 Thalassomonas sp. LD5

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Thalassomonas sp. LD5

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 45 more than 50% of the enzyme activity is lost below 30°C and above 45°C Thalassomonas sp. LD5

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 40 the enzyme retains 100% or 60% activity after being incubated at 10-20°C or 30°C for 1 h, respectively. The enzyme loses nearly 95% activity after being incubated at 40°C for 1 h Thalassomonas sp. LD5

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Thalassomonas sp. LD5