Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Pachysandra terminalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
1 * 53000 + 1 * 22000, SDS-PAGE | Pachysandra terminalis |
53000 | - |
1 * 53000 + 1 * 22000, SDS-PAGE | Pachysandra terminalis |
70000 | - |
gel filtration | Pachysandra terminalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pachysandra terminalis | E3PQS3 | Japanese spurge | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pachysandra terminalis |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, concanavalin A column chromatography, Mono S (pH 4.5) column chromatography and Mono S (pH 4.0) column chromatography | Pachysandra terminalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | in vitro incubations with sucrose as a single substrate show a prominent formation of 6-kestotriose compared to 1-kestotriose and 6G-kestotriose (neokestose) and the formation of 1- and 6-kestotetraose from sucrose and 1-kestotriose | Pachysandra terminalis | D-fructose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 53000 + 1 * 22000, SDS-PAGE | Pachysandra terminalis |
Synonyms | Comment | Organism |
---|---|---|
6-SST | - |
Pachysandra terminalis |
fructan 6-exohydrolase | - |
Pachysandra terminalis |
suc:fructan 6-fructosyltransferase | - |
Pachysandra terminalis |
sucrose:fructan 6-fructosyltransferase | - |
Pachysandra terminalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
about 20°C | Pachysandra terminalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 45 | - |
Pachysandra terminalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Pachysandra terminalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | - |
Pachysandra terminalis |