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Literature summary for 3.2.1.154 extracted from

  • Tamura, K.I.; Sanada, Y.; Tase, K.; Komatsu, T.; Yoshida, M.
    Pp6-FEH1 encodes an enzyme for degradation of highly polymerized levan and is transcriptionally induced by defoliation in timothy (Phleum pratense L.) (2011), J. Exp. Bot., 62, 3421-3431.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.154

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X-33 Phleum pratense

Inhibitors

Inhibitors Comment Organism Structure
sucrose the inhibitory effect of sucrose is 4% at 10 mM sucrose and 23% at 100 mM sucrose Phleum pratense

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
 Phleum pratense 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63400
-
 x * 63400, putative mature protein, calculated from amino acid sequence Phleum pratense
78000
-
 x * 78000, recombinant enzyme, SDS-PAGE Phleum pratense

Organism

Organism UniProt Comment Textmining
Phleum pratense E9RGV6 cultivar Hokushu
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has five N-glycosylation sites Phleum pratense

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Phleum pratense
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,1-kestopentaose + H2O 18% activity compared to levan Phleum pratense ?
-
 ?
1,1-kestotetraose + H2O 18% activity compared to levan Phleum pratense ?
-
 ?
1-kestotriose + H2O 7% activity compared to levan Phleum pratense ?
-
 ?
6-kestotriose + H2O 25% activity compared to levan Phleum pratense ?
-
 ?
inulin + H2O 6% activity compared to levan Phleum pratense ?
-
 ?
levan + H2O 100% activity, the enzyme shows 6-FEH activity against levan (mean degree of polymerization is 20) that is 4fold higher than against 6-kestotriose(mean degree of polymerization is 3) Phleum pratense ?
-
 ?
additional information the enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans and shows weak invertase activity Phleum pratense ?
-
 ?
sucrose + H2O 15% activity with 100 mM sucrose and less than 1% activity with 1 mM sucrose compared to levan Phleum pratense D-fructose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 63400, putative mature protein, calculated from amino acid sequence Phleum pratense
? x * 78000, recombinant enzyme, SDS-PAGE Phleum pratense

Synonyms

Synonyms Comment Organism
6-FEH1
-
 Phleum pratense
fructan 6-exohydrolase
-
 Phleum pratense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 about 30°C Phleum pratense

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 30 the activity of the enzyme remains relatively high even at 4°C, with up to 38% of its activity at 30°C Phleum pratense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Phleum pratense

pI Value

Organism Comment pI Value Maximum pI Value
Phleum pratense putative mature protein, calculated from amino acid sequence
-
 5

Expression

Organism Comment Expression
Phleum pratense in the stubble of seedlings and in young haplocorms from adult timothy plants, transcripts of 6-FEH1 are significantly increased (about 7fold) within 3 h of defoliation, followed by an increase in fructan 6-exohydrolase activity up

General Information

General Information Comment Organism
physiological function the enzyme plays a role in the degradation of fructans and the mobilization of carbon sources for regrowth after defoliation in timothy Phleum pratense