Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta-gami 2 cells | Chondrostereum purpureum |
Protein Variants | Comment | Organism |
---|---|---|
A365P | the mutant shows about 70% of wild type activity | Chondrostereum purpureum |
D395N | the mutant shows about 10% of wild type activity | Chondrostereum purpureum |
E364Q | the mutant shows about 20% of wild type activity | Chondrostereum purpureum |
E364Q/E366Q | the mutant shows about 60% of wild type activity | Chondrostereum purpureum |
H150A | the mutant shows about 20% of wild type activity | Chondrostereum purpureum |
H195A | inactive | Chondrostereum purpureum |
H98A | inactive | Chondrostereum purpureum |
K205A | inactive | Chondrostereum purpureum |
K228A | inactive | Chondrostereum purpureum |
K253A | the mutant shows about 50% of wild type activity | Chondrostereum purpureum |
K318A | inactive | Chondrostereum purpureum |
K88A | the mutant shows about 1% of wild type activity | Chondrostereum purpureum |
P339A | the mutant shows about 5% of wild type activity | Chondrostereum purpureum |
P348A | the mutant shows about 80% of wild type activity | Chondrostereum purpureum |
P352A | the mutant shows about 35% of wild type activity | Chondrostereum purpureum |
P355A | the mutant shows about 30% of wild type activity | Chondrostereum purpureum |
P358A | the mutant shows about 7% of wild type activity | Chondrostereum purpureum |
R220A | the mutant shows about 10% of wild type activity | Chondrostereum purpureum |
R226A | inactive | Chondrostereum purpureum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chondrostereum purpureum | P79074 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and DE52-cellulose column chromatography | Chondrostereum purpureum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.33 | - |
crude extract, at pH 4.5 and 30°C | Chondrostereum purpureum |
435 | - |
after 187fold purification, at pH 4.5 and 30°C | Chondrostereum purpureum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Chondrostereum purpureum | ? | - |
? | |
polygalacturonate + H2O | - |
Chondrostereum purpureum | oligogalacturonates | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Chondrostereum purpureum |
Synonyms | Comment | Organism |
---|---|---|
EndoPG I | - |
Chondrostereum purpureum |
endopolygalacturonase I | - |
Chondrostereum purpureum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Chondrostereum purpureum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | about 35% activity at 40°C, about 75% activity at 50°C, 100% activity at 60°C, about 60% activity at 70°C, about 45% activity at 80°C | Chondrostereum purpureum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme activity remains stable (more than 90% activity) after 30 min between 30 and 60°C | Chondrostereum purpureum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.4 | - |
Pectin | pro-enzyme, at pH 4.5 and 30°C | Chondrostereum purpureum | |
20.2 | - |
Pectin | pro-enzyme, at pH 4.0 and 30°C | Chondrostereum purpureum | |
230 | - |
Pectin | mature enzyme, at pH 4.5 and 30°C | Chondrostereum purpureum | |
334 | - |
Polygalacturonate | pro-enzyme, at pH 4.5 and 30°C | Chondrostereum purpureum | |
996 | - |
Polygalacturonate | mature enzyme, at pH 4.5 and 30°C | Chondrostereum purpureum | |
1670 | - |
Polygalacturonate | pro-enzyme, at pH 4.0 and 30°C | Chondrostereum purpureum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Chondrostereum purpureum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | the enzyme activity remains stable after 16 h between pH 3.0 and 7.0 | Chondrostereum purpureum |