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Literature summary for 3.2.1.15 extracted from

  • Abdulrachman, D.; Thongkred, P.; Kocharin, K.; Nakpathom, M.; Somboon, B.; Narumol, N.; Champreda, V.; Eurwilaichitr, L.; Suwanto, A.; Nimchua, T.; Chantasingh, D.
    Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring (2017), BMC Biotechnol., 17, 15 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain KM71 Aspergillus aculeatus

Protein Variants

Protein Variants Comment Organism
industry the enzyme is suitable for application as a textile bioscouring agent Aspergillus aculeatus

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ 73.76% residual activity at 1 mM Aspergillus aculeatus
additional information EDTA has no significant effect on the enzyme activity Aspergillus aculeatus
Na+ 90.88% residual activity at 1 mM Aspergillus aculeatus
Zn2+ 88.81% residual activity at 1 mM Aspergillus aculeatus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 112.7% activity at 1 mM Aspergillus aculeatus
Fe2+ 112.03% activity at 1 mM Aspergillus aculeatus
additional information Ca2+, K+, Li+, and Mg2+ have no significant effect on the enzyme activity Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
-
-
Aspergillus aculeatus ATCC16872
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin + H2O 25% activity with 6-7% esterified pectin and 22% activity with 55-70% esterified pectin, compared to polygalacturonate Aspergillus aculeatus D-(+)-galacturonic acid + ?
-
 ?
citrus pectin + H2O 25% activity with 6-7% esterified pectin and 22% activity with 55-70% esterified pectin, compared to polygalacturonate Aspergillus aculeatus ATCC16872 D-(+)-galacturonic acid + ?
-
 ?
additional information the enzyme shows no non-specific hydrolysis activity against Avicel, soluble starch or carboxymethyl cellulose Aspergillus aculeatus ?
-
 ?
additional information the enzyme shows no non-specific hydrolysis activity against Avicel, soluble starch or carboxymethyl cellulose Aspergillus aculeatus ATCC16872 ?
-
 ?
polygalacturonate + H2O 100% activity Aspergillus aculeatus oligogalacturonates
-
 ?
polygalacturonate + H2O 100% activity Aspergillus aculeatus ATCC16872 oligogalacturonates
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Aspergillus aculeatus
? x * 38700, calculated from amino acid sequence Aspergillus aculeatus

Synonyms

Synonyms Comment Organism
endo-polygalacturonase
-
 Aspergillus aculeatus
endoPG
-
 Aspergillus aculeatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus aculeatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80 more than 50% activity between 30 and 80°C Aspergillus aculeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme retains more than 60% of its initial activity after incubation at 40°C for 180 min. The enzyme rapidly loses activity at 50°C and 60°C Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus aculeatus

pH Range

pH Minimum pH Maximum Comment Organism
4 5.5 more than 50% activity at pH 4.0-5.5 Aspergillus aculeatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 9 more than 80% of enzyme activity is retained after incubation in pH 2.0 to 6.0 for 24 h. A marked decrease in enzyme activity is observed at pH 7.0 to 9.0 Aspergillus aculeatus