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Literature summary for 3.2.1.15 extracted from

  • Tu, T.; Li, Y.; Luo, Y.; Wang, Z.; Wang, Y.; Luo, H.; Yao, B.
    A key residue for the substrate affinity enhancement of a thermophilic endo-polygalacturonase revealed by computational design (2018), Appl. Microbiol. Biotechnol., 102, 4457-4466 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells Bispora sp. MEY-1

Protein Variants

Protein Variants Comment Organism
D129K the mutant shows increased catalytic efficiency towards trigalacturonic acid compared to the wild type enzyme Bispora sp. MEY-1
D129K/D214N inactive Bispora sp. MEY-1
D129R the mutant shows about wild type activity and stability Bispora sp. MEY-1

Organism

Organism UniProt Comment Textmining
Bispora sp. MEY-1 A0A2R4K332
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein three glycosylation sites at Asn181, Asn201 and Asn282 Bispora sp. MEY-1

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q Sepharose column chromatography Bispora sp. MEY-1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonate + H2O
-
Bispora sp. MEY-1 D-(+)-galacturonate
-
?
trigalacturonic acid + H2O
-
Bispora sp. MEY-1 ?
-
?

Subunits

Subunits Comment Organism
? x * 37400, calculated from amino acid sequence Bispora sp. MEY-1
? x * 38000, deglycosylated enzyme, SDS-PAGE Bispora sp. MEY-1
? x * 50000, glycosylated enzyme, SDS-PAGE Bispora sp. MEY-1

Synonyms

Synonyms Comment Organism
endo-PG
-
Bispora sp. MEY-1
endo-polygalacturonase
-
Bispora sp. MEY-1
PG28A
-
Bispora sp. MEY-1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Bispora sp. MEY-1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 80 the enzyme shows more than 60% activity between 60 and 80°C Bispora sp. MEY-1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
the enzyme retains more than 90% activity after 30 min at 60°C Bispora sp. MEY-1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
Bispora sp. MEY-1

pH Range

pH Minimum pH Maximum Comment Organism
2.5 4.5 the enzyme shows more than 60% activity between pH 2.5 and 4.5 Bispora sp. MEY-1

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.2 7 the enzyme retains more than 90% activity after 1 h at pH 2.2-7.0 and 37°C Bispora sp. MEY-1

pI Value

Organism Comment pI Value Maximum pI Value
Bispora sp. MEY-1 calculated from amino acid sequence
-
4.3