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Literature summary for 3.2.1.15 extracted from

  • Tu, T.; Luo, H.; Meng, K.; Cheng, Y.; Ma, R.; Shi, P.; Huang, H.; Bai, Y.; Wang, Y.; Zhang, L.; Yao, B.
    Improvement in thermostability of an Achaetomium sp. strain Xz8 endopolygalacturonase via the optimization of charge-charge interactions (2015), Appl. Environ. Microbiol., 81, 6938-6944 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Achaetomium sp. Xz8

Protein Variants

Protein Variants Comment Organism
D244A the mutant shows significantly increased thermostability and retains activity comparable to that of the wild type enzyme Achaetomium sp. Xz8
D244A/D299R the mutant shows catalytic activity, which is comparable to that of the wild type enzyme. The mutant shows the most pronounced shifts in temperature of maximum enzymatic activity, temperature at which 50% of the maximal activity of an enzyme is retained, and melting temperature, of about 10, 17, and 10.2°C upward, respectively, with the half-life extended by 8.4 h at 50°C and 45 min at 55°C compared to the wild type Achaetomium sp. Xz8
D299R the mutant shows significantly increased thermostability and retains activity comparable to that of the wild type enzyme Achaetomium sp. Xz8

Organism

Organism UniProt Comment Textmining
Achaetomium sp. Xz8
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonate + H2O
-
 Achaetomium sp. Xz8 D-galacturonate
-
 ?

Synonyms

Synonyms Comment Organism
endopolygalacturonase
-
 Achaetomium sp. Xz8
PG8fn
-
 Achaetomium sp. Xz8

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Achaetomium sp. Xz8

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 50% activity after 1.6 h at 50°C, 50% activity after 33 min at 55°C, 96% loss of activity after 30 min at 80°C Achaetomium sp. Xz8

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
 Achaetomium sp. Xz8