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Literature summary for 3.2.1.15 extracted from

  • Kusuma, M.; Sri Rami Reddy, D.
    Purification and characterization of polygalacturonase using isolated Bacillus subtilis C4 (2014), Res. J. Microbiol., 9, 95-103.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid
-
Bacillus subtilis
L-cysteine
-
Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
Bacillus subtilis
Mn2+
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus subtilis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates slightly at 10 mM Bacillus subtilis
Mg2+ activates slightly at 10 mM Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis C4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 15fold by ammonium sulfate fractionation, dialysis, anion exchange and affinity chromatography Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining
additional information submerged fermentation on pectin Bacillus subtilis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13790
-
purified enzyme, pH 9.0, 60°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Bacillus subtilis ?
-
?
amylopectin + H2O
-
Bacillus subtilis C4 ?
-
?
dextrose agar + H2O potato dextrose agar Bacillus subtilis ?
-
?
dextrose agar + H2O potato dextrose agar Bacillus subtilis C4 ?
-
?
pectin + H2O high activity Bacillus subtilis ?
-
?
pectin + H2O high activity Bacillus subtilis C4 ?
-
?
polygalacturonic acid + H2O best substrate Bacillus subtilis galacturonic acid
-
?
polygalacturonic acid + H2O best substrate Bacillus subtilis C4 galacturonic acid
-
?

Subunits

Subunits Comment Organism
? x * 43000-66000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
alkaline endo-PG
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90 activity range Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 purified enzyme, pH 9.0, 60 min, stable Bacillus subtilis
60
-
purified enzyme, pH 9.0, 60 min, loss of 80% activity Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
3 10 activity range Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
purified enzyme, 60°C, 60 min, stable Bacillus subtilis