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Literature summary for 3.2.1.15 extracted from
- Purification and kinetic studies of polygalacturonase from Aspergillus niger RBF96 (2014), J. Pure Appl. Microbiol., 8, 2389-2394.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | polygalacturonases are pectin substances degrading enzymes, that are widely used in juice and fruit beverages for quality improvement | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.155 | - |
Pectin | pH 4.5, 50°C | Aspergillus niger |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | Aspergillus niger | citrus or apple pectin | ? | - |
? | |
| pectin + H2O | Aspergillus niger RBF96 | citrus or apple pectin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger RBF96 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially 7fold by gel filtration | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 83.06 | - |
partially purified enzyme, pH 4.5, 50°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | citrus or apple pectin | Aspergillus niger | ? | - |
? | |
| pectin + H2O | citrus or apple pectin | Aspergillus niger RBF96 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aspergillus niger |
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Release 2023.1 (January 2023)
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