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Literature summary for 3.2.1.15 extracted from

  • Tu, T.; Meng, K.; Bai, Y.; Shi, P.; Luo, H.; Wang, Y.; Yang, P.; Zhang, Y.; Zhang, W.; Yao, B.
    High-yield production of a low-temperature-active polygalacturonase for papaya juice clarification (2013), Food Chem., 141, 2974-2981.
    View publication on PubMed
Search for more literature for 3.2.1.15

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates by 24.5% Achaetomium sp. Xz-8

Application

Application Comment Organism
food industry the enzyme reduces the viscosity of papaya juice by 17.6% and increases its transmittance by 59.1%. Its favourable enzymatic properties make the enzyme attractive for potential applications in the juice industry Achaetomium sp. Xz-8

Cloned(Commentary)

Cloned (Comment) Organism
gene pg I, DNA and amino acid sequence determination and analysis, overexpression in Pichia pastoris in fed-batch fermentation Achaetomium sp. Xz-8

Inhibitors

Inhibitors Comment Organism Structure
Ag+ over 50% inhibition Achaetomium sp. Xz-8
Ca2+ over 50% inhibition Achaetomium sp. Xz-8
Cr3+ below 40% inhibition Achaetomium sp. Xz-8
Cu2+ below 40% inhibition Achaetomium sp. Xz-8
EDTA below 40% inhibition Achaetomium sp. Xz-8
Mg2+ below 40% inhibition Achaetomium sp. Xz-8

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35400
-
 x * 35400, about, sequence calculation, x * 36900, about, mass spectrometry Achaetomium sp. Xz-8
36900
-
 x * 35400, about, sequence calculation, x * 36900, about, mass spectrometry Achaetomium sp. Xz-8

Organism

Organism UniProt Comment Textmining
Achaetomium sp. Xz-8 S5MK25 isolated from a desert sand sample of Ningxia, China, gene pg I
-
Achaetomium sp. Xz-8 Xz8 S5MK25 isolated from a desert sand sample of Ningxia, China, gene pg I
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation Achaetomium sp. Xz-8

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris strain GS115 by ultrafiltration, gel filtration, and anion exchange chromatography, followed by deglycosylation of the proteins by endo-beta-N-acetylglucosaminidase H Achaetomium sp. Xz-8

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28120
-
 purified recombinant enzyme, pH 6.0, 45°C Achaetomium sp. Xz-8

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information endo-PG I is a polygalacturonase of endo-type Achaetomium sp. Xz-8 ?
-
 ?
additional information endo-PG I is a polygalacturonase of endo-type Achaetomium sp. Xz-8 Xz8 ?
-
 ?
pectin + H2O citrus pectin Achaetomium sp. Xz-8 ?
-
 ?
pectin + H2O citrus pectin Achaetomium sp. Xz-8 Xz8 ?
-
 ?
polygalacturonic acid + H2O
-
 Achaetomium sp. Xz-8 digalacturonic acid + galacturonic acid + trigalacturonic acid
-
 ?
polygalacturonic acid + H2O
-
 Achaetomium sp. Xz-8 Xz8 digalacturonic acid + galacturonic acid + trigalacturonic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 35400, about, sequence calculation, x * 36900, about, mass spectrometry Achaetomium sp. Xz-8

Synonyms

Synonyms Comment Organism
endo-PG I
-
 Achaetomium sp. Xz-8
endo-polygalacturonase
-
 Achaetomium sp. Xz-8

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Achaetomium sp. Xz-8

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80 activity range Achaetomium sp. Xz-8

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 45 purified recombinant enzyme, pH 6.0, 1 h without substrate, completely stable Achaetomium sp. Xz-8
55
-
 purified recombinant enzyme, pH 6.0, 1 h without substrate,loss of 70% activity Achaetomium sp. Xz-8

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Achaetomium sp. Xz-8

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 40% of maximal activity within this range Achaetomium sp. Xz-8

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 8 purified recombinant enzyme, 37°C, 1 h without substrate, over 75% activity remaining Achaetomium sp. Xz-8

General Information

General Information Comment Organism
evolution the enzyme belongs to the family 28 of glycosyl hydrolases Achaetomium sp. Xz-8
physiological function the enzyme reduces the viscosity of papaya juice by 17.6% and increases its transmittance by 59.1%. The enzyme works synergistically with pectin methylesterase Achaetomium sp. Xz-8