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Literature summary for 3.2.1.15 extracted from
- Production, purification, and characterization of polygalacturonase from Rhizomucor pusillus isolated from decomposting orange peels (2012), Enzyme Res., 2012, 138634.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Rhizomucor pusillus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
1 * 32000, SDS-PAGE | Rhizomucor pusillus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid + H2O | Rhizomucor pusillus | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizomucor pusillus | - |
isolated from decomposting orange peels | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 12.34fold by two different steps of gel filtration, to homogeneity | Rhizomucor pusillus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
32.57 U/ml for crude enzyme and 61.35 U/ml for purified enzyme, pH 5.0, 55°C | Rhizomucor pusillus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | 37.8% activity compared to polygalacturonic acid | Rhizomucor pusillus | ? | - |
? | |
| pectin + H2O | 57% activity compared to polygalacturonic acid | Rhizomucor pusillus | ? | - |
? | |
| polygalacturonic acid + H2O | - |
Rhizomucor pusillus | ? | - |
? | |
| polygalacturonic acid + H2O | best substrate | Rhizomucor pusillus | ? | - |
? | |
| xylan + H2O | 11% activity compared to polygalacturonic acid | Rhizomucor pusillus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE | Rhizomucor pusillus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PGase | - |
Rhizomucor pusillus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Rhizomucor pusillus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 27% and 13% of maximal activity at 30°C and 70°C, resepctively | Rhizomucor pusillus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, pH 4.0-5.0, 120 min, stable, rapid inactivation above | Rhizomucor pusillus |
| 60 | - |
above, purified enzyme, pH 5.0, 48.2% activity remaining after 10 min | Rhizomucor pusillus |
| 70 | - |
above, purified enzyme, pH 5.0, 9.3% activity remaining after 10 min | Rhizomucor pusillus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Rhizomucor pusillus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
above, purified enzyme, 60°C, rapid inactivation | Rhizomucor pusillus |
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