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Literature summary for 3.2.1.15 extracted from

  • Eschstruth, A.; Divol, B.
    Comparative characterization of endo-polygalacturonase (Pgu1) from Saccharomyces cerevisiae and Saccharomyces paradoxus under winemaking conditions (2011), Appl. Microbiol. Biotechnol., 91, 623-634.
    View publication on PubMed
Search for more literature for 3.2.1.15

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, overexpression in Saccharomyces cerevisiae strain VIN13, which is itself completely devoid of endo-polygalacturonase activity, and secretion to the medium Saccharomyces paradoxus
overexpression in Saccharomyces cerevisiae strain VIN13, which is itself completely devoid of endo-polygalacturonase activity, and secretion to the medium Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P47180 gene PGU1
-
Saccharomyces cerevisiae BY4742 P47180 gene PGU1
-
Saccharomyces paradoxus F5B9B9 gene PGU1
-
Saccharomyces paradoxus RO88 F5B9B9 gene PGU1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonic acid + H2O
-
 Saccharomyces cerevisiae galacturonic acid + ?
-
 ?
polygalacturonic acid + H2O
-
 Saccharomyces paradoxus galacturonic acid + ?
-
 ?
polygalacturonic acid + H2O
-
 Saccharomyces cerevisiae BY4742 galacturonic acid + ?
-
 ?
polygalacturonic acid + H2O
-
 Saccharomyces paradoxus RO88 galacturonic acid + ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-polygalacturonase
-
 Saccharomyces cerevisiae
endo-polygalacturonase
-
 Saccharomyces paradoxus
pectinase
-
 Saccharomyces cerevisiae
pectinase
-
 Saccharomyces paradoxus
Pgu1
-
 Saccharomyces cerevisiae
Pgu1
-
 Saccharomyces paradoxus
ScPgu1
-
 Saccharomyces cerevisiae
SpPgu1
-
 Saccharomyces paradoxus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Saccharomyces cerevisiae
45
-
-
 Saccharomyces paradoxus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25% of maximal activity at 25°C, maximal activity at 45°C Saccharomyces paradoxus
25 45 30% of maximal activity at 25°C, maximal activity at 45°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Saccharomyces cerevisiae
5.5
-
-
 Saccharomyces paradoxus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 4.5 79% of maximal activity at pH 3.5, maximal activity at pH 4.5 Saccharomyces cerevisiae
3.5 5.5 43% of maximal activity at pH 3.5, maximal activity at pH 5.5 Saccharomyces paradoxus

General Information

General Information Comment Organism
additional information comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions Saccharomyces cerevisiae
additional information comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions Saccharomyces paradoxus