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Literature summary for 3.2.1.15 extracted from

  • Buga, M.; Ibrahim, S.; Nok, A.
    Physico-chemical characteristics of immobilized polygalacturonase from Aspergillus niger (SA6) (2010), Afr. J. Biotechnol., 9, 8934-8943.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the partially purified native enzyme, the sodium alginate immobilized polygalacturonase exhibits more stability to changes in pH than the temperature. Activity of the immobilized polygalacturonase is reduced to 34.56% and 14.81% of the initial activity after the second and third catalytic cycles, respectively, half-life is 10 min at pH 4.5, 40°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, SDS-PAGE Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger SA6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 9fold by ultrafiltration, ammonium sulfate fractionation, ion exchange chromatography, and gel filtration Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
246
-
partially purified enzyme, pH 4.5, 40°C Aspergillus niger

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
endo PG
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Aspergillus niger