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Literature summary for 3.2.1.15 extracted from
- Thermal and high-pressure stability of purified polygalacturonase and pectinmethylesterase from four different tomato processing varieties (2006), Food Res. Int., 39, 440-448.
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | high pressure processing can be used for selective inactivation of PG in tomato processing while keeping pectinmethylesterase intact | Solanum lycopersicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | PG1 and PG2 can be inactivated at room temperature in the pressure range of 300-500 MPa | Solanum lycopersicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
43000, SDS-PAGE, 43700, Maldi-Tof-spec analysis, PG1 and PG2 | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | - |
varieties Galeon, Malpica, Perfectpeel and Soto | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by cation exchange chromatography | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | ripe tomato. Varieties Galeon and Soto have about 50% of each enzyme, whereas Malpica and Perfectpeel contain more PG1 than PG2 | Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid + H2O | - |
Solanum lycopersicum | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 43000, SDS-PAGE, 43700, Maldi-Tof-spec analysis, PG1 and PG2 | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PG1 | - |
Solanum lycopersicum |
| PG2 | - |
Solanum lycopersicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
inactivation at around 55°C corresponds to PG2 and at around 80°C corresponds to PG1. After 5 min at 90°C almost complete inactivation | Solanum lycopersicum |
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