Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | strongly inhibits PG1 and PG2 | Athelia rolfsii | |
Cr3+ | stronger inhibitor for PG2 than for PG1 | Athelia rolfsii | |
Fe3+ | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii | |
Hg2+ | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii | |
Mg2+ | - |
Athelia rolfsii | |
additional information | EDTA and surfactants have no influence on enzyme activity | Athelia rolfsii | |
SDS | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
PG2 | Athelia rolfsii |
39500 | - |
PG1 | Athelia rolfsii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Athelia rolfsii | - |
- |
- |
Athelia rolfsii CBS 350.80 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, by free flow electrophoresis in isoelectric focusing mode using precipitation, followed by gel filtration | Athelia rolfsii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity of PG1 and PG2 towards xylan, carboxymethylcellulose and mannan | Athelia rolfsii | ? | - |
? | |
additional information | no activity of PG1 and PG2 towards xylan, carboxymethylcellulose and mannan | Athelia rolfsii CBS 350.80 | ? | - |
? | |
pectin + H2O | citrus pectin with degree of esterification of 63-66%, bad substrate for PG1 and PG2. Apple pectin with degree of esterification of 70-75%, bad substrate for PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
pectin + H2O | citrus pectin with degree of esterification of 63-66%, bad substrate for PG1 and PG2. Apple pectin with degree of esterification of 70-75%, bad substrate for PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
polygalacturonic acid + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
polygalacturonic acid + H2O | PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
[beta-D-GalA(1-4)]3 + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
[beta-D-GalA(1-4)]3 + H2O | PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
[beta-D-GalA(1-4)]4 + H2O | only cleaved by PG1 and PG2 when trigalacturonic acid is present | Athelia rolfsii | ? | - |
? | |
[beta-D-GalA(1-4)]4 + H2O | only cleaved by PG1 and PG2 when trigalacturonic acid is present | Athelia rolfsii CBS 350.80 | ? | - |
? | |
[beta-D-GalA(1-4)]5 + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PG1 | - |
Athelia rolfsii |
PG2 | - |
Athelia rolfsii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
isoenzyme PG2 | Athelia rolfsii |
60 | - |
isoenzyme PG1 | Athelia rolfsii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
PG1 is stable at 50°C, at 70°C stability is drastically reduced. PG2 is a very sensitive enzyme, its half-life time at 50°C is in the range of min and at 70°C the enzyme is inactivated within 1 min | Athelia rolfsii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
isoenzyme PG2 | Athelia rolfsii |
5 | - |
isoenzyme PG1 | Athelia rolfsii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
PG1 is stable at pH 5, exhibits more sensitivity against pH values below and above 5 | Athelia rolfsii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Athelia rolfsii | PG2 | - |
5.4 |
Athelia rolfsii | PG1 | - |
6.5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.07 | - |
isoenzyme PG1 | Athelia rolfsii | Hg2+ | |
0.5 | - |
isoenzyme PG1 | Athelia rolfsii | SDS | |
0.75 | - |
isoenzyme PG1 | Athelia rolfsii | Fe3+ | |
0.9 | - |
isoenzyme PG2 | Athelia rolfsii | Hg2+ | |
5 | - |
isoenzyme PG1 and PG2 | Athelia rolfsii | Mg2+ | |
7 | - |
isoenzyme PG1 | Athelia rolfsii | Ca2+ | |
12 | - |
isoenzyme PG2 | Athelia rolfsii | SDS | |
15 | - |
isoenzyme PG2 | Athelia rolfsii | Ca2+ | |
16 | - |
isoenzyme PG2 | Athelia rolfsii | Fe3+ | |
18 | - |
isoenzyme PG2 | Athelia rolfsii | Cr3+ | |
32 | - |
isoenzyme PG1 | Athelia rolfsii | Cr3+ |