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Literature summary for 3.2.1.15 extracted from

  • Voget, C.E.; Vita, C.E.; Contreras Esquivel, J.C.
    One-step concentration and partial purification of Aspergillus kawachii non-acidic polygalacturonases by adsorption to glass fiber microfilters (2006), Biotechnol. Lett., 28, 233-239.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
gel filtration, PG5 fraction PGIII Aspergillus luchuensis
38000
-
gel filtration, PG5 fraction PGII Aspergillus luchuensis
41000
-
41000, SDS-PAGE, PG5 fraction PGII Aspergillus luchuensis

Organism

Organism UniProt Comment Textmining
Aspergillus luchuensis
-
-
-
Aspergillus luchuensis IFO 4308
-
-
-

Purification (Commentary)

Purification (Comment) Organism
one-step concentration and partial purification of PG5 by adsorption to glass fiber microfilters, 100fold. By gel filtration two PG5 fractions (PGII and PGII), 306fold Aspergillus luchuensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15
-
PG5 in fermentation broth Aspergillus luchuensis
1506
-
100fold purified PG5 Aspergillus luchuensis
4585
-
306fold purified PG5 fraction PGII Aspergillus luchuensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonic acid + H2O
-
Aspergillus luchuensis ?
-
?
polygalacturonic acid + H2O
-
Aspergillus luchuensis IFO 4308 ?
-
?

Subunits

Subunits Comment Organism
monomer 41000, SDS-PAGE, PG5 fraction PGII Aspergillus luchuensis

Synonyms

Synonyms Comment Organism
endo-PG
-
Aspergillus luchuensis
non-acidic polygalacturonase
-
Aspergillus luchuensis
PG5
-
Aspergillus luchuensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
optimum pHs for PG5 fractions PGII and PGIII towards polygalacturonic acid are 4.7 and 5, respectively Aspergillus luchuensis

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
PG5 activity in the permeate decreases, as compared to fermentation broth, at pH values higher than 2.5 being negligible at pH 5.0 Aspergillus luchuensis

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus luchuensis PG5 fraction PGII
-
5.3