Application | Comment | Organism |
---|---|---|
nutrition | potential application of the enzyme in fruit juice extraction | Thermothelomyces heterothallicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Thermothelomyces heterothallicus | |
Ca2+ | 5 mM | Thermothelomyces heterothallicus | |
Cu2+ | 5 mM | Thermothelomyces heterothallicus | |
Mg2+ | strong inhibition | Thermothelomyces heterothallicus | |
SDS | - |
Thermothelomyces heterothallicus | |
Triton X-100 | slight inhibition | Thermothelomyces heterothallicus | |
Tween 80 | slight inhibition | Thermothelomyces heterothallicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the KM-value for pectin is 0.416 mg/ml | Thermothelomyces heterothallicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, stimulates | Thermothelomyces heterothallicus | |
Cu2+ | 1 mM, stimulates | Thermothelomyces heterothallicus | |
Fe2+ | 1 mM and 5 mM, stimulates | Thermothelomyces heterothallicus | |
Mn2+ | 1 mM and 5 mM, stimulates | Thermothelomyces heterothallicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces heterothallicus | - |
Apinis | - |
Thermothelomyces heterothallicus Apinis | - |
Apinis | - |
Purification (Comment) | Organism |
---|---|
- |
Thermothelomyces heterothallicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Thermothelomyces heterothallicus | ? | - |
? | |
pectin + H2O | - |
Thermothelomyces heterothallicus Apinis | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Thermothelomyces heterothallicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | 50°C: about 55% of maximal activity, 70°C: about 50% of maximal activity | Thermothelomyces heterothallicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
t1/2: 4 h | Thermothelomyces heterothallicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermothelomyces heterothallicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | pH 4.0: about 50% of maximal activity, pH 8.0: about 50% of maximal activity | Thermothelomyces heterothallicus |