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Literature summary for 3.2.1.15 extracted from
- New polygalacturonases from Trichoderma reesei: characterization and their specificities to partially methylated and acetylated pectins (2003), Carbohydr. Res., 338, 515-524.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
PG2, gel filtration | Trichoderma reesei |
| 66000 | - |
PG1, gel filtration | Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | - |
ATCC 26920 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trichoderma reesei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Trichoderma reesei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | the two enzymes PG1 and PG2 have higher affinity towards polygalacturonate derived from sugar beet pectin than polygalacturonate derived from lime pectin. Enzymatic activity decreases with increased degree of methylation of lime pectins | Trichoderma reesei | oligogalacturonides | - |
? |  |
| polygalacturonate + H2O | the two enzymes PG1 and PG2 have higher affinity towards polygalacturonate derived from sugar beet pectin than polygalacturonate derived from lime pectin | Trichoderma reesei | oligogalacturonides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 66000, PG1 or PG2, SDS-PAGE | Trichoderma reesei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PG1 | - |
Trichoderma reesei |
| PG2 | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
PG1 | Trichoderma reesei |
| 50 | - |
PG2 | Trichoderma reesei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | 20°C: about 60% of maximal activity, 50°C: about 90% of maximal activity, 60°C: about 10% of maximal activity, PG1 | Trichoderma reesei |
| 30 | 60 | 30°C: about 60% of maximal activity, 60°C: about 75% of maximal activity, 70°C: about 25% of maximal activity, PG2 | Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
15 min, PG1 loses about 30% of its activity, PG2 loses about 10% of its activity | Trichoderma reesei |
| 50 | - |
15 min, PG1 loses about 80% of its activity, PG2 loses about 10% of its activity | Trichoderma reesei |
| 60 | - |
15 min, PG1 loses about 90% of its activity, PG2 loses about 20% of its activity | Trichoderma reesei |
| 70 | - |
15 min, PG1 loses about 95% of its activity, PG2 loses about 85% of its activity | Trichoderma reesei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
PG2 | Trichoderma reesei |
| 4.5 | - |
PG1 | Trichoderma reesei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | 4.5 | pH 3.8: about 80% of maximal activity, pH 4.5: about 50% of maximal activity, PG2 | Trichoderma reesei |
| 3.8 | 4.9 | pH 3.8: about 40% of maximal acticity, pH 4.9: about 50% of maximal activity, PG1 | Trichoderma reesei |
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Release 2023.1 (January 2023)
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