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Literature summary for 3.2.1.15 extracted from
- Production, purification, and characterization of a polygalacturonase from a new strain of Kluyveromyces marxianus isolated from coffee wet-processing wastewater (2002), Appl. Biochem. Biotechnol., 97, 193-208.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
polygalacturonic acid | - |
Kluyveromyces marxianus |  |
| 48.8 | - |
Pectin | apple pectin, 75% degree of esterification | Kluyveromyces marxianus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41700 | - |
x * 41700, SDS-PAGE | Kluyveromyces marxianus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Kluyveromyces marxianus | O2 and galacturonate negatively regulate enzyme synthesis, and glucose as carbon source affords better enzyme yield than lactose | ? | - |
? | |
| additional information | Kluyveromyces marxianus CCEBI 2011 | O2 and galacturonate negatively regulate enzyme synthesis, and glucose as carbon source affords better enzyme yield than lactose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | - |
- |
- |
| Kluyveromyces marxianus CCEBI 2011 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Kluyveromyces marxianus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 219 | - |
- |
Kluyveromyces marxianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | O2 and galacturonate negatively regulate enzyme synthesis, and glucose as carbon source affords better enzyme yield than lactose | Kluyveromyces marxianus | ? | - |
? | |
| additional information | O2 and galacturonate negatively regulate enzyme synthesis, and glucose as carbon source affords better enzyme yield than lactose | Kluyveromyces marxianus CCEBI 2011 | ? | - |
? | |
| pectin + H2O | apple pectin, 75% degree of esterification | Kluyveromyces marxianus | ? | - |
? | |
| pectin + H2O | apple pectin, 75% degree of esterification | Kluyveromyces marxianus CCEBI 2011 | ? | - |
? | |
| polygalacturonate + H2O | - |
Kluyveromyces marxianus | ? | - |
? | |
| polygalacturonate + H2O | - |
Kluyveromyces marxianus CCEBI 2011 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41700, SDS-PAGE | Kluyveromyces marxianus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PG | - |
Kluyveromyces marxianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Kluyveromyces marxianus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
14 h, 50% loss of activity | Kluyveromyces marxianus |
| 55 | - |
8.7 h: 50% loss of activity | Kluyveromyces marxianus |
| 60 | - |
complete inactivation after 6 min | Kluyveromyces marxianus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.83 | - |
polygalacturonic acid | - |
Kluyveromyces marxianus | |
| 6.08 | - |
Pectin | apple pectin, 75% degree of esterification | Kluyveromyces marxianus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Kluyveromyces marxianus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4.5 | pH 3.0: about 50% of maximal activity, pH 4.5: optimum | Kluyveromyces marxianus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | stable | Kluyveromyces marxianus |
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