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Literature summary for 3.2.1.15 extracted from
- Purification and characterization of an endo-polygalacturonase from a mutant of Saccharomyces cerevisiae (1999), Biosci. Biotechnol. Biochem., 63, 1100-1103.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
gel filtration | Kluyveromyces marxianus |
| 44000 | - |
1 * 44000, SDS-PAGE | Saccharomyces cerevisiae |
| 45000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | - |
- |
- |
| Saccharomyces cerevisiae | - |
SSM52 | - |
| Saccharomyces cerevisiae SSM52 | - |
SSM52 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | putative glycosylation sites at Asn294 and Asn306 | Saccharomyces cerevisiae |
| glycoprotein | partially glycosylated | Kluyveromyces marxianus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Saccharomyces cerevisiae | oligogalacturonates | - |
? |  |
| polygalacturonate + H2O | - |
Kluyveromyces marxianus | oligogalacturonates | - |
? | |
| polygalacturonate + H2O | - |
Saccharomyces cerevisiae SSM52 | oligogalacturonates | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 44000, SDS-PAGE | Saccharomyces cerevisiae |
| monomer | 1 * 41000-43000, SDS-PAGE | Kluyveromyces marxianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Saccharomyces cerevisiae |
| 60 | - |
- |
Kluyveromyces marxianus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | 30°C: about 55% of maximal activity, 55°C: about 60% of maximal activity | Saccharomyces cerevisiae |
| 30 | 65 | 30°C: about 45% of maximal activity, 65°C: about 50% of maximal activity | Kluyveromyces marxianus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Saccharomyces cerevisiae |
| 5 | - |
- |
Kluyveromyces marxianus |
| 6 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | pH 4.0: about 35% of maximal activity, pH 7.0: about 80% of maximal activity | Saccharomyces cerevisiae |
| 4 | 7 | pH 4.0: about 70% of maximal activity, pH 7.0: about 80% of maximal activity | Kluyveromyces marxianus |
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Release 2023.1 (January 2023)
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