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Literature summary for 3.2.1.15 extracted from

  • Lim, J.; Yamasaki, Y.; Suzuki, Y.; Ozawa, J.
    Multiple forms of endo-polygalacturonase from Saccharomyces fragilis (1980), Agric. Biol. Chem., 44, 473-480.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ HgCl2 Saccharomyces fragilis
Mn2+ MnCl2 Saccharomyces fragilis
PCMB
-
Saccharomyces fragilis
Tris
-
Saccharomyces fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, isoenzyme III, SDS-PAGE Saccharomyces fragilis
46000
-
x * 46000, isoenzyme I, SDS-PAGE Saccharomyces fragilis
50000
-
x * 50000, isoenzyme II, SDS-PAGE Saccharomyces fragilis

Organism

Organism UniProt Comment Textmining
Saccharomyces fragilis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isoenzyme I contains 0.8% mannose, isoenzyme II contains 0.9% mannose, isoenzyme III contains 3.7% mannose. The neutral sugar of the three enzymes is mainly mannose Saccharomyces fragilis

Purification (Commentary)

Purification (Comment) Organism
isoenzyme I, II and III Saccharomyces fragilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces fragilis
130
-
isoenzyme II Saccharomyces fragilis
134.3
-
isoenzyme III Saccharomyces fragilis
145
-
isoenzyme I Saccharomyces fragilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information macerating activity towards potato and carrot tissues Saccharomyces fragilis ?
-
?
pectic acid + H2O
-
Saccharomyces fragilis galacturonic acid oligomers
-
?
polygalacturonate + H2O
-
Saccharomyces fragilis oligogalacturonates
-
?

Subunits

Subunits Comment Organism
? x * 50000, isoenzyme II, SDS-PAGE Saccharomyces fragilis
? x * 46000, isoenzyme I, SDS-PAGE Saccharomyces fragilis
? x * 30000, isoenzyme III, SDS-PAGE Saccharomyces fragilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Saccharomyces fragilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 about 60% of maximal activity at 40°C and at 60°C Saccharomyces fragilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 5.0, 15 min, stable up to Saccharomyces fragilis
50
-
pH 5.0, 15 min, complete loss of activity Saccharomyces fragilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
Saccharomyces fragilis
5
-
-
Saccharomyces fragilis

pH Range

pH Minimum pH Maximum Comment Organism
3 5 pH 3.0: about 40% of maximal activity, pH 5.0: about 50% of maximal activity Saccharomyces fragilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 6 30°C, 20 h, stable Saccharomyces fragilis