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Literature summary for 3.2.1.15 extracted from
- Purification, crystallization and some properties of endopolygalacturonase from Kluyveromyces fragilis (1984), Agric. Biol. Chem., 48, 1951-1961.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Saccharomyces fragilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Saccharomyces fragilis |  |
| Ba2+ | - |
Saccharomyces fragilis | |
| Ca2+ | - |
Saccharomyces fragilis | |
| Hg+ | - |
Saccharomyces fragilis | |
| Hg2+ | - |
Saccharomyces fragilis | |
| Pb2+ | - |
Saccharomyces fragilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
gel filtration | Saccharomyces fragilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | - |
- |
- |
| Kluyveromyces wickerhamii | - |
- |
- |
| Saccharomyces fragilis | - |
i.e. Kluyveromyces fragilis | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Kluyveromyces marxianus |
| glycoprotein | contains 3.2% sugar | Saccharomyces fragilis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces fragilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces fragilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Saccharomyces fragilis | oligogalacturonates | - |
? | |
| polygalacturonate + H2O | - |
Kluyveromyces marxianus | oligogalacturonates | - |
? | |
| polygalacturonate + H2O | - |
Kluyveromyces wickerhamii | oligogalacturonates | - |
? | |
| protopectin + H2O | - |
Saccharomyces fragilis | highly polymerized pectin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Saccharomyces fragilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 5.0, stable up to | Saccharomyces fragilis |
| 55 | - |
30 min, 50% loss of activity | Saccharomyces fragilis |
| 70 | - |
30 min, complete loss of activity | Saccharomyces fragilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | 37°C, 30 min, stable | Saccharomyces fragilis |
| 5 | - |
30°C, stable | Saccharomyces fragilis |
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